Can fusarium cultures be preserved as glycerol stocks for long term preservation purpose in the same way as used for bacterial cultures?

More Kamesh Krishnamoorthy Kalyankumar's questions See All

What does an "Error 111" mean on an AKTA Explorer?

Dear Colleagues, I would be grateful if any of you can advise me on what an "error 111" is on an AKTA Explorer. The manual suggests that the instrument be turned off and turned on. That does not...

13 June 2024 2,961 1 View

Where do I obtain Chenopodium quinoa Willd. (quinoa) seeds (SAMN04338310; QQ74) that were originally used for genome sequencing?

The Chenopodium quinoa Willd. (quinoa) seeds with accession PI614886 (Biosample accession code SAMN04338310; also known as NSL 106399 and QQ74) is what I require and was also used for genome...

10 December 2023 5,680 2 View

What dilutions of serum sample should I use in ELISA?

I would like to do an ELISA to measure the levels of a biomarker in mouse serum. The concentration of the standards ranges from 2000pgs/ml to 31.25pgs/ml. I have the kit from R&D systems but...

18 February 2022 10,013 2 View

Sequence identity matrix?

While generating a sequence identity matrix in bioedit software by pasting the sequences from notepad, often there is an overlapping of sequence names (given before the fasta sequence) and the...

10 January 2020 1,339 0 View

Between Julia and MATLAB, which would be the most efficient for a discontinuous Galerkin FEM code?

I need to simulate a 2-D problem with DGFEM. I have been advised to use C/C++ for a code that delivers quick and efficient results, however, I am not very comfortable with them, and my base code...

16 December 2019 7,151 5 View

Is the Zero Shot Learning a good current research area or not?

I am going to do my research on Zero Shot Learning. Is it good area in recent trends...

03 December 2018 7,235 3 View

Increased manganese availability in flooded soils?

plants like paddy dont suffer from manganese deficiency since they are grown in flooded soils? what is the reason for the increased availability of manganese in flooded soils?

14 April 2018 6,544 2 View

First aid measure for Ethidium bromide spill?

If there is an accidental ethidium bromide spill on skin/hands during a moelcular work what is the immediate first aid measure to be taken using very simple chemicals available in the lab?

12 March 2018 443 3 View

Any simple protocol for whitefly DNA extraction?

Iam finding it hard to get amplification post whitefly DNA extraction using lysis buffer method. Any other simple methodology. What are the precautions or troubleshooting measures to be followed?...

09 March 2018 3,232 4 View

Insects for DNA extraction stored in ethanol post collection?

Generally insects like whiteflies collected for DNA extraction are stored in ethanol. Does ethanol act as a preservant. Does it help in maintaining the integrity of the insect DNA. How long till...

17 February 2018 667 2 View

Why Do TDS and EC Increase with Larger Wastewater Volumes, While BOD and COD Decrease?

I have carried out MFC experiments on three different volumes, 50, 500 and 1000 mL of wastewater. Results after MFC treatment shows that TDS and EC are more in larger volumes of water i.e. TDS and...

09 August 2024 9,621 0 View

Could it be a cell culture contamination?

Hello everyone! I observed in my culture (htert-RPE1 cells) an orange- red particle at the bottom of the dish. It is visible to the naked eye as a very very small red dot. Could it be a...

09 August 2024 2,824 3 View

Does Anyone have expertise in in vitro transcription and RNA pull down assay?

I am currently working on LncRNA; to know the lncRNA-protein interactions I want to do RNA pull down assay, so I need to design primers with T7 promoter. I need assistance in this regard.

07 August 2024 6,622 1 View

Is there any way to quantify bacterial and fungal cells in their mixed culture?

I am working in fungal fermentation of soybean meal and there is bacterial growth in them at times. I am trying to quantify fungal cell counts and bacterial cells; but I haven't been able to do at...

07 August 2024 7,535 4 View

How to normalize and take the significance of the MTT OD values with 3 replicates for the same cell-line?

Hi, I have a question about normalizing the MTT OD values for doing the statistical analysis. So, if we have 3 different plates and we call them 3 different replicates, so, first we would...

07 August 2024 8,106 4 View

Why activated CAR-Jurkat cell could not kill targets?

Previously when I co-coluture anti-CD19(FMC63) CAR-Jurkat with Raji with E:T=5:1, Jurkat can eliminate Raji in 24h. However, when I test another CAR construct, although I can dectect totally CD69...

06 August 2024 641 2 View

RNA later for the preservation of RNA in fecal samples at room temperature for one day (37°C)?

I am planning to collect human fecal samples for metatranscriptomic analysis using MGI. These samples are from indigenous people living in a region with high temperatures. I will have access to a...

06 August 2024 1,367 3 View

Do you have good tips for seaweed tissue preservation in the field for post RNA extraction?

I will be with my students collecting seaweed samples in a marine farm and later we will process this tissue for RNA isolation and further sequencing. Does anyone have tips on how to collect the...

04 August 2024 501 2 View

How to isolate lymphocytes from mouse spleen?

I have tried several times to isolate lymphocytes from mouse spleen, but all attempts have been unsuccessful. I tried most available protocols. I used different dissociation media (HBSS with Ca...

04 August 2024 9,913 7 View

How to Add Missing Water Molecules in Protein-Membrane Simulations?

I have protein-membrane simulations (PDB, PSF, DCD) and have noticed that water molecules near the protein are not visible in the simulations. How can I fix this issue? Is there a way to place the...

04 August 2024 1,200 2 View