I am attempting to prepare proteoliposome and have some specific questions.
1) Can EYPC made liposomes be stored at 4 degree in buffer MES ph 6.0, 50mM MgCl2, 100mM KCl ?
2) Can I use superfine G 100 column for proteoliposome separation from empty liposome, and protein? for 200nm proteoliposome (500uL) what may be an approximate bed volume? or other specifications like flow rate?
Thanks