Hi everyone,

Reaching out today to see if you can help me with my research project.

Lately, we're focusing on enzymatic synthesis. But I don't have a biologist background, rather analytical chemist one, so I am struggling with one question that came out a few months ago. We would like to find a way to first, detect and then, quantify the enzymes in the final product, to know if there are traces of enzymes in it.

The idea was then to try and dissolve the powder-form enzymes used as "catalyst" and developed a HPLC-UV and/or HPLC-MS (ToF) from this solution, before analyzing the final product. However, it was not conclusive (no peak in the chromatogram; and when there's a peak, m/z ratio are hard to exploit = is it the enzyme, a degraded version of it, something else that keeps the enzyme in powder form...?).

I am pretty sure I am not having the correct way of thinking this problem through.

So, shoud you have any experience in this field, I could really use some research data (articles, technical notes...) or any tips on how to proceed for this specific subject.

Thanks in advance for your time and help!

Célia