How to best prepare cells for FACS from live tissue?

Hi everyone, I'm currently trying to perform single cell RNA seq on a small (

24 January 2021 9,804 2 View

How do I post details of my new book?

Barracks, Forts and Ramparts: Regeneration Challenges for Portsmouth Harbour's Defence Heritage, by Celia Clark with Martin Marks OBE. Tricorn Books 2020. ISBN 9781912821648. 450 pages,...

30 December 2020 2,872 1 View

Titulo: Recombinase Polymerase Amplification (RPA) assay design?

23 November 2020 7,770 3 View

Has anyone stained pSTAT3 in T cells for FACS?

I am stimulating with IL-27, IL-6, or IL-21 and trying to see pSTAT3 in naive CD4+ T cells. I have tried BD's protocol using the Perm buffer III, I have used Stumhofer et al., 2007, AOH Hall et...

03 November 2020 2,653 3 View

Which solvent would you choose to perform polyamide-6 dip-coating on metal part ?

Which solvent would you choose to perform polyamide 6 coating on metal part ? I guess the vapor pressure at room temperature has to be high to evaporate properly after dipping. So among m-cresol,...

11 October 2020 8,104 3 View

Can the magnetic permeability and electric permittivity of the vacuum be modeled as the quantum vacuum magnetization and polarization, respectively?

If the magnetic field permeability and electric field permittivity of the quantum vacuum at a point in spacetime may be understood as the effective vacuum magnetization and vacuum polarization,...

04 June 2020 7,655 2 View

Solutions for arc faults on the DC side of a PV installation (Solar installation)?

Hello everyone, I am working on PV projects in the Netherlands and as in Europe it is not mandatory to install specific protection devices for arc faults there is not much info about this, I was...

25 May 2020 9,773 3 View

Is there any free, open source, softweare available to convert Agilent GC-MS (*.D) files to ANDI/AIA or JCAMP-DX?

Dear all, I am trying to convert some large Agilent GC-MS files (*.D) to a standard format such as ANDI/AIA (*.CDF) or JCAMP-DX (*.JDX). I was wondering if any one knows a piece of software that...

19 February 2020 8,289 3 View

When coal fired power plants are eliminated, what will be the source of fly ash for concrete?

Fly ash is used in concrete to help eliminate the alkali - silica reactivity between portland cement and the surface of the aggregate. Alkali - Silica reactivity can turn concrete highways into...

27 January 2020 6,027 3 View

Papers that accept discovery studies?

Hello colleagues, I was wondering if I could ask for general advice or guidance on something? I tried to submit a paper recently, but it got rejected on this occasion. Essentially, we did a...

26 November 2019 3,921 2 View

Is there any published or unpublished statistics on dropout rate (Dose1 to Dose-2) for Covid-19 vaccination in India, US, UK or any other country?

Are there any published or unpublished statistics or papers on the dropout rate (Dose1 to Dose-2) for Covid-19 vaccination in India, USA, UK, or any other country where vaccination has started at...

28 February 2021 4,237 1 View

Please suggest the website or link for the collection of data for (Cancer Incidance Rates in India)?

We are doing research for the prediction of cancer incidance rates increases in India upto next five years. Please help us to get the exact data for Cancer Incidance Rates in India. This will...

19 February 2021 8,478 2 View

Access to supercomputer resources in India?

Hello everyone, I want to perform some gaussian calculations and want to use Materials studio for my research work. Can anyone guide me how to get access to supercomputer resources in India for...

15 February 2021 8,264 3 View

What is India's InternationalTrade Policy during Pendamic? How do you find the policy as successful one?

During Covid-19 Pendamic Government are facing problem to maintain business. India is one of them so this question is perfect to be discussed here.

08 February 2021 2,725 3 View

Which satellite image gives best results for Land Use land cover classification(specifically for built up regions) for Indian region ?

Landsat, sentinel and USGS satellite images have varying accuracy for identification of urban built up dynamics ? Which one is best suited for India

04 February 2021 8,632 8 View

What is the role of IQAC to improve the grading of an institution?

IQAC provides all information about institution and its various activities to NAAC, Bangluru, India in order to get good grading. In this light what is the role of IQAC??

03 February 2021 6,916 1 View

I want some expert (free) advice and guidance in interpreting depositional environment from well logs in a deltaic setting. Anyone ready to help?

I am looking into the geophysical well logs from a coaliferous basin in India. I need some help in depicting the depositional environment from well logs.

02 February 2021 6,052 3 View

Can any tell me or suggest any research article or review about the concentration of peptide dissolve in system water and given to Adult Zebrafish?

Hello, everyone! I have chemically synthesized antioxidant peptide isolated from the plant. I have to validate its bioactivities in Adult zebrafish. I have to administer this peptide to zebra...

01 February 2021 5,809 3 View

How to measure volume of Pinus roxburghii ?

I want to measure volume of Pinus roxburghii in the Jammu region of India to calculate the carbon stocks of Pinus roxburghii.

31 January 2021 2,311 3 View

Sample Size for Quasi-Experimental Quants using one-tailed t-test?

Hi, sorry I'm stumped. I'm going to do a study on the leadership styles (using the MLQ Form 5X) of minority women administrators in medical schools. How will I calculate the sample size? Thank...

30 January 2021 2,865 4 View