Novel delivery system of a VEGF antagonist using encapsulated cells for tumor therapy are explored. HPI-1, is a potent antagonist of the Hh transcription factor Gli1 and functions independent of upstream components in the pathway. Systemic administration of HPI-1 ineeds minimal aqueous solubility but show poor bioavailability. However, polymeric nanoparticle from [poly(lactic-co-glycolic acid); (PLGA)] conjugated with polyethylene glycol (PEG), encapsulating HPI-1 (NanoHHI). NanoHHI particles have an average diameter of approximately 60 nm, forms uniform aqueous suspension are engineered. These have improved systemic bioavailability compared with the parent compound. In contrast to the prototype targeted Smo antagonist.

Microencapsulation can be done with 2% alginate, to which is dissolved by stirring the alginate in warm water. Subsequently, it is mixed with the microorganism, and the mixture is homogenized. This preparation is dropped drop by drop into a solution of 1N calcium chloride. The reaction is allowed to last for 15 minutes and filtered to remove the microcapsules themselves wrapped and left to dry Whatman 1 filter paper for 12 hr.

To recover the organism, it takes a particle and seeded in culture medium was regularly used. The microparticles are hard and do not dissolve. The microorganism is released only in the culture medium. (for alginate methodology Quim. Nova, Vol. 33, No. 7, 1435-1439, 2010)

Than you for your useful idea and information. I have ever worked with alginate for my Talaromyces flavus, a fungus but how can it work for bacteria antagonis that do not have endospore? How can the bacteria live for long time in the formula?

Yes the bacteria Live for ten Years  or more in alginate microcapsules