Can E. coli One Shot Mach1 T1 still be used if stored at -20°C?

More Bertrand Chesneau's questions See All

How can I build a membrane patch with multiple monomers of a peptide?

Hi, I have been looking around for ways to insert various monomers of an AMP in membrane bilayers. To start-off, I have been using Charm-Gui, but it seems that I can only insert one peptide at a...

29 November 2020 8,660 2 View

Which tests for a quantitative experimental design using scenario with formative constructs?

Hello! I am lost as to know which tests I should use. I am doing a master thesis in marketing/IS. I have two groups (treatment received used AI) and control (did not use AI). I used an...

27 April 2019 4,317 2 View

Is there any study or research paper that deals with the disturbances faced by insects in the natural conditions?

There are countless research papers dealing with the menace and destructive nature of insects. In a way, insects are unfortunately deemed as detrimental organisms of the planet as there are...

23 July 2018 2,305 2 View

Is there a standard protocol to look for the parts of insects in the faecal matter of insectivorous birds?

The reliance of birds over insects for nutrition is an interesting ecological phenomenon. The reliance varies from being obligatory to facultative, but the major chunk of bird population are some...

25 June 2018 6,219 3 View

Which type of spectrotometry method is apt for analysing the pesticide trace contents in insects?

Rapid usage of pesticides in agriculture has gained its way into the body of insects. I intend to check the content present from my personal collection of insects like grasshoppers and beetles...

06 March 2018 1,705 7 View

Which parameters could be measured to prevent an epilepsy seizure ?

I'm looking for chemical or physiological parameters (which can be measured) that change minutes or seconds before an epilepsy crisis. In other terms, what are the chemical, biological and...

11 October 2014 8,274 6 View

Comparison of the intensities of low-loss and core loss EELS spectra acquired with different exposure times and vertical binning?

Recently I acquired two EELS spectrum images (low-loss and core-loss on the N-K edge) over cavities that contains molecular nitrogen (using a GIF Quantum). I have extracted the signature of the...

30 September 2014 2,202 2 View

Are there any good tools for determining or predicting dimer or multimer formation for large proteins?

I'm currently sequencing laccase isoforms. I basically have the sequences. However I would like to predict the formation of multimers due to the fact that this phenomena occurs frequently in...

18 February 2014 6,275 1 View

What features/factors would you look for in an Atomic Layer Deposition (ALD) Reactor?

As we have been spreading the word about ALD and its usefulness we have found many people who haven't heard of the technique. We are working on putting together an economical ALD reactor that is...

03 October 2012 3,198 18 View

Alternative to mediation analysis?

I am currently working on my dissertation and want to explore the role of competence as a mediator between the effect of colour of clothes on voting intention (focus on Western countries)....

21 February 2021 8,609 1 View

How COVID-19 has affected mental health?

The pandemic has led to depression and anxiety and its negative effects shouldn't be ignored. However, it has also led some people to become more resilient! What do you think? Thank you.

21 February 2021 4,733 19 View

What new can be added in the field of historical studies to implement more realistic programs?

What new can be added in the field of historical studies to implement more realistic programs in dealing with the events of history and interpreting them with scientific sincerity and competence?

26 January 2021 559 1 View

COVID-19: Individual level, cross-country Panel dataset. Which method to use?

I am struggling with the most appropriate method to use for my Master thesis on the impact on mental health indicators of informal caregivers before and after the start of the COVID-19 pandemic. I...

07 January 2021 1,456 5 View

What are the mental health insurance policies in different countries?

Post Covid 19 the scenario in India has changed and some of the private insurance companies are providing coverages for mental disorders. So My question is what mental disorders are covered in...

04 January 2021 6,474 10 View

Did you know trees in cities can lower stress and boost our mental health?

Benefits of urban trees :

22 December 2020 9,702 2 View

What are the competencies to put forward in Higher Education to prepare students to evolve in a world where IA is ubiquituous?

15 December 2020 6,350 4 View

How Healthcare Personnel help frontline workers cope with stress and mental burden during COVID-19?

Which methods does they suggest to ease the burden and stress as a teamwork?

09 December 2020 9,605 7 View

What's the analysis I need follow in SPSS step by step ? What statistical test I will use to find sample size using Gpower software?

Hello, I am conducting an experiment for my dissertation and looking at the effect that relaxing photos have at mood , physical and mental health. There is only one group that is going to be used...

23 November 2020 7,471 3 View

Are you a virtual team member/leader who would like to participate in a Delphi study on International Online Collaboration Competencies (IOCC)?

You are invited to participate as expert panel member in our consensus study on International Online Collaboration Competencies (IOCC). The purpose of this study is to build consensus on the key...

11 November 2020 9,715 1 View

María García-Portela

Hi Bertrand,

More than an answer this is a question since I have the same problem. Did you finally use them? If so, how did it go? Thanks in advance!

Bertrand Chesneau

Hi María,

Sorry for the delay in responding.

We used them to amplify a vector for which we had already made a construction and it worked. It's worth a try.

I hope it works for you too.