In short, yes.

Digital PCR allows for very accurate detection and quantification of DNA solutions and is independent of a calibrant.

The Limit of Detection for PCR is one molecule of the target DNA sequence. As such, Digital PCR allows you to count the number of molecules present in a solution that is partitioned into a large number of individual reactions. As long as the number and volume of the partitions are known, you can determine the number of copies per µL relatively easily.

Have a read of the paper I linked to, as well as some of the others below (ResearchGate only lets me link to one publication at a time).

Also see:

https://www.researchgate.net/publication/51832041_Evaluation_of_a_droplet_digital_polymerase_chain_reaction_format_for_DNA_copy_number_quantification?ev=prf_pub

https://www.researchgate.net/publication/45535288_Comparison_of_methods_for_accurate_quantification_of_DNA_mass_concentration_with_traceability_to_the_international_system_of_units?ev=prf_pub

https://www.researchgate.net/publication/26882551_Absolute_quantification_of_genetically_modified_MON810_maize_%28Zea_mays_L.%29_by_digital_polymerase_chain_reaction?ev=prf_pub

Article Single molecule detection in nanofluidic digital array enabl...

Article Evaluation of a Droplet Digital Polymerase Chain Reaction Fo...

Article Comparison of Methods for Accurate Quantification of DNA Mas...

Article Absolute quantification of genetically modified MON810 maize...

Dear Jacob

Thank you for the competent answer and publications.

best regards

René köppel