I did an experiment which involved adding DHA to a cancer cell line. Most research suggests that the cells should be affected by its addition at the concentrations I used. However the cells did not show any affect to it. Could this be because DHA was not dissolved or taken up by cells: it was dissolved in PBS, what should have been used?
Ideally, you should use human albumin as a carrier for PUFA (e.g. DHA), it is more physiologically.
Hi,
After a quick search, it seems to me that DHA is recommended to be dissolved in sterile absolute ethyl alcohol and stored at -20°C.
For more information, please consult the following link:
Docosahexaenoic acid (DHA), an omega-3 fatty acid, inhibits tumor growth and metastatic potential of ovarian cancer - PMC (nih.gov)
Best regards
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