How to use RNALater with bacterial cultures?

02 March 2017 714 7 View

Fast Cloning, Help to understand the method of primes?

01 February 2017 9,883 7 View

What is the procedure for carrying out a Relative quantitative real time PCR?

I need to verify a microarray data for top 5 genes using Relative quantitative Real time per (using delta delta Ct method) I have no previous knowledge to run the experiment. Could somebody...

05 June 2014 3,169 3 View

Can anyone help with transcriptional and translational fusion?

For transcriptional fusion do you clone the promoter of interest upstream of the reporter gene which is promoterless to study the promoter strength? And in translational fusion do you clone the...

03 April 2014 368 7 View

TA cloning problem?

Something went wrong! I have a PCR product purified from gel by qiagen kit. Then Add A" overhang as following (7.6 purified PCR product, 1ul of 10X buffer, 0.2 ul of 10mM dNTP and 0.2 ul DynaZyme...

10 November 2013 7,356 13 View

How to calculate the concentration of PCR product from DNA mass standard on gel (e.g.1kb from neb) for sequencing reaction?

I need to use 1 ul of 3-10 ng of my pcr product to sequence by BigDye 3.1 protocol. How can i measure the concentration of my pcr product out of the gel ladder/standard?

10 November 2013 9,023 10 View

Overlapping PCR?

I need help with overlapping PCR, I have 4 primers A, B, C and D where B and C have complementary bases. 1. first PCR run with Phusion Polymerase from Thermo F-530S to generate PCR product AB and...

10 November 2013 1,858 15 View

What is the difference between Gene Cluster and Operon in bacteria

Gene cluster vs operon

03 April 2013 770 8 View

error in running a model in Abaqus

Excessive rotation of nodes in node set ErrNodeExcessRotation-Step1

17 August 2021 0 0 View

error in modelling model in Abaqus

Excessive rotation of nodes in node set ErrNodeExcessRotation-Step1

17 August 2021 0 0 View

MD MOE RMSD

How to calculate the RMSD values for a MD simulation using MOE?

07 August 2021 0 0 View

MD MOE RMSD

How to calculate the RMSD values for a MD simulation using MOE?

07 August 2021 0 0 View

MGL tools

20 June 2021 0 0 View

fatal error

When I tried to energy minimization my system, I got fatal error as below. Fatal error: Atomtype opls_116 not found Although I've already added this line: ; include water #include "oplsaa.ff/spc.itp" to [molecultype] directive in my topology.

16 June 2021 0 0 View

fatal error

When I tried to energy minimization my system, I got fatal error as below. Fatal error: Atomtype opls_116 not found Although I've already added this line: ; include water #include "oplsaa.ff/spc.itp" to [molecultype] directive in my topology.

16 June 2021 0 0 View

fatal error

When I tried to energy minimization my system, I got fatal error as below. Fatal error: Atomtype opls_116 not found Although I've already added this line: ; include water #include "oplsaa.ff/spc.itp" to [molecultype] directive in my topology.

16 June 2021 0 0 View

fatal error

When I tried to energy minimization my system, I got fatal error as below. Fatal error: Atomtype opls_116 not found Although I've already added this line: ; include water #include "oplsaa.ff/spc.itp" to [molecultype] directive in my topology.

16 June 2021 0 0 View

what is the simplest definition of ion exchange capacity

What this unit identifies for the ion exchange capacity ca. 0.92 meq. g-1. I am a newcomer in the membrane area. I just started. I highly appreciate your help

08 May 2021 0 0 View