9 Questions 19 Answers 0 Followers
Questions related from Miriam Khider
Dear, I am working with a slow growing bacteria. I need to harvest 10 ml at the low OD and approximately 5ml at high OD for RNA isolation and transcriptomics. I saw that most people use RNA...
03 March 2017 781 7 View
I am doing this for the first time and I need a quick check wether i have understood the method correctly. My plasmid has a CAM gene which is going to be deleted and replaced with KAN. 1. Primer...
02 February 2017 9,963 7 View
I need to verify a microarray data for top 5 genes using Relative quantitative Real time per (using delta delta Ct method) I have no previous knowledge to run the experiment. Could somebody...
06 June 2014 3,226 3 View
Can Colony PCR be applied to genomic DNA, or only for ligation (vector/insert) screening?
06 June 2014 8,934 7 View
For transcriptional fusion do you clone the promoter of interest upstream of the reporter gene which is promoterless to study the promoter strength? And in translational fusion do you clone the...
04 April 2014 428 7 View
I need to use 1 ul of 3-10 ng of my pcr product to sequence by BigDye 3.1 protocol. How can i measure the concentration of my pcr product out of the gel ladder/standard?
11 November 2013 9,118 10 View
Something went wrong! I have a PCR product purified from gel by qiagen kit. Then Add A" overhang as following (7.6 purified PCR product, 1ul of 10X buffer, 0.2 ul of 10mM dNTP and 0.2 ul DynaZyme...
11 November 2013 7,396 13 View
I need help with overlapping PCR, I have 4 primers A, B, C and D where B and C have complementary bases. 1. first PCR run with Phusion Polymerase from Thermo F-530S to generate PCR product AB and...
11 November 2013 1,927 15 View
Gene cluster vs operon
04 April 2013 937 8 View
