Is there a typical range in the power spectrum that each person has during a rest measurement? Moreover, are there differences between men and women?

Is there a typical range in the power spectrum that each person has in the individual brain areas (19 electrodes, 10-20 system) and frequency bands (theta, alpha, beta gamma) during a rest...

13 January 2021 5,660 3 View

How can I find electricity generation data of small to medium scale PV panels and Wind turbines?

I am using model predictive control to simulate market market participation of a Virtual Power Plant in the north of the Netherlands. The power plant portfolio consists of local (geographical...

21 September 2020 4,409 11 View

Does anyone have experience with nattokinase, or another way to dissolve fibrogen?

We want to extract RNA from a 3D TC and nattokinase was suggested, but i am not sure whats the best supplier - dissolving technique, and amount and incubation time to add to the 3D TC ?

01 September 2020 7,424 3 View

How to separate monocytes by plastic adhesion?

07 July 2020 5,883 10 View

Does anyone know what kind of insect this is?

The flower was found from a Hydrangea species in my garden. These little bugs were found hiding within the flower. Do you know what they are?

11 August 2019 3,037 8 View

Does anyone have a precise protocol for NK cytotoxiciy assay based on calcein release?

I'm currently searching for natural killer cell cytotoxicity tests that are not based on flow cytometry but on calcein release measured in the supernatant. Can anyone help with protocols?

19 May 2019 2,514 1 View

Can I calculate the coherence of two EEG signals on a given frequency band or do I have to calculate them over the entire frequency range?

I would like to know whether I first have to calculate the coherence between two signals over the entire frequency spectrum in matlab and summarize the specific frequency bands from these values...

09 May 2019 9,695 2 View

Cell line recommendations for transfection of plasmid vector with human insert?

I have constructed a plasmid with a human DNA insert (5kb) that has the luciferase reporter gene. I will be looking to transfect this plasmid into mammalian cells and run a luciferase assay to...

25 January 2019 3,991 3 View

How to optimise PCR for linearising 4.2kb plasmid?

I'm trying to linearise a plasmid that I will use as my vector for NEBuilder HiFi DNA Assembly. I have designed primers for linearising the plasmid that I am now testing. There are shorter bands...

04 December 2018 8,935 3 View

Has anyone used a Vglu2 anti mouse antibody on mouse brain?

18 October 2016 3,905 3 View

Enhanced Yellow Fluorescent Protein (Aequorea victoria) DNA sequence?

I am on the lookout for the Enhanced Yellow Fluorescent Protein (Aequorea victoria) DNA sequence. Does anyone know where I can find it? Thank you in advance

03 March 2021 3,568 1 View

What element is driving the different slop in the low frequency tail?

My study consists simple corrosion inhibitors in salty brine. Right now I just fit my circuit with randles with CPE. Just wondering how the slop can given more info of the experiment. Or any...

01 March 2021 6,474 3 View

Why might my GFP tag be dissociating from my plasmid after transfection?

I have a set of stably transfected cell lines all transfected with plasmids containing GFP tags on the C terminus. During a western blot using anti-GFP antibody, one of my plasmids has dissociated...

01 March 2021 9,310 4 View

Which fluorescent dye to use for measuring total ROS production?

Is DCFDA appropriate for measuring total ROS production, or can it only detect certain types of ROS? If I want to measure total ROS production, which dye should I use?

01 March 2021 9,447 3 View

Precipitate after adding laemmli buffer to protein solution containing Potassium Acetate?

Hi, I am running a size exclusion chromatography experiment with a buffer containing Potassium Acetate as a salt. I analyse these fractions through SDS-PAGE. After boiling my SEC fractions in...

01 March 2021 2,622 3 View

What is the most suitable server for predicting protein structures in bacteria?

I have used the i-Tasser several times, however it has been unavailable for several days. I tried the swiss-model, but the output was not very pleasant due to the model used. Is there any other...

28 February 2021 4,521 3 View

Do you need the CMV enhancer before the CMV promotor for a proper function of CMV on the gene that follows?

I'm a student and I have to produce a cell line with a knockin for the NRF2 gene with GFP. I have to put a promotor in front of the GFP because the gene will be too far away from the promotor of...

28 February 2021 7,127 2 View

Are protein samples in SDS compatible with ELISA?

I have used an AllPrep DNA/RNA/Protein Mini Kit QIAGEN kit to extract RNA and protein from my samples. At the end of the protein purification, I resuspend my protein in 5% SDS. Will these samples...

28 February 2021 7,370 3 View

The reason that the culture of white fungi Contamination with green mold(Trichoderma), the culture autoclave at121 for 15 min. What is it?

Is the period to autoclave not enough? The inoculation in a hood and flame and UV and alcohol.

27 February 2021 9,356 3 View

No amplification data for qPCR on MxPro?

I have set up a qPCR run on MxPro with SYBR green and ROX as a reference, however, when I go to the analysis section the program seems to only collect data for the dissociation curves and not the...

25 February 2021 8,039 1 View