I am synthesizing CDNA using the BioRad iscript CDNA synthesis kit. The protocol says to 1. heat the samples to 250C 5min (Priming)

2. 46 0C for 20 min (Reverse transcription)

3. 95 0C for 1min (RT inactivation)

4. hold the samples in 4 0C

During the synthesis process, first step and the second step was done but my PCR machine failed at the 3rd step, so it did not heat samples to 950C 1min. I immediately moved the samples to 40C (on ice) and had to store the samples in -200C, but can this CDNA samples be saved without the RT inactivation step? Will it be ok if I re-run them for the last step?