W.K.C. Udeesha Erandani

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Can CDNA synthesis process fail due to the RT inactivation step failure?

I am synthesizing CDNA using the BioRad iscript CDNA synthesis kit. The protocol says to 1. heat the samples to 250C 5min (Priming) 2. 46 0C for 20 min (Reverse transcription) 3. 95 0C for 1min...

13 March 2024 1,251 2 View