The OVCAR-3 medium suggested by ATCC is RPMI-1640 with 0.01 mg/ml bovine insulin and 20% FBS. I would like to replace bovine insulin for human recombinant insulin. Has anyone tried this? Thanks in advance!
Hello all, I am preparing an experiment to evaluate chromatin accessibility (ATAC-SeqAssay for Transposase-Accessible Chromatin using sequencing) in a mouse model. Since the number of cells needed...
07 May 2023 1,041 0 View
Dear Professor/Professors I'm sorry to trouble, with this thing tha might be so easy, I thought I knew but somehow I confused myself For instance, I have a 100 ml solution of CuO and DI water...
16 February 2023 5,534 1 View
I need a reference about the methodology. How it was developed? When it was used for the first time?
26 January 2020 6,024 2 View
Hi! It is very similar to Nanodrop. It worked very well, however, the display doesn't turn on today. Do you know this equipament? Anyone can help me, please? Thanks!
20 November 2019 4,032 3 View
Hi, I'm not biogeochemistry specialist. This isn't my area. So this question may seem stupid to some. I would like to know if there may be excess biological nitrogen fixation and if this carries...
05 March 2018 7,469 7 View
Hello everyone, I am trying to purify my protein (enzyme) expressed in Pichia pastoris through ion exchange chromatography. The protein is extracellular, so I extract the supernatant after...
27 July 2017 4,167 5 View
My negative control (only cells non stimulated stained with FLICA) is stained as my positive control (LPS+MSU cystals). Does anyone have any idea what should I do in order to avoid it? The...
20 November 2016 5,771 4 View
This is a schist, the mineral is uniaxic (+) but I doubt that it is quartz since there are others that show that typical clean surface different from this one that exhibits lots of inclusions, can...
27 August 2016 688 16 View
I have a question about the mediation effect/ analysis from this data. What is important to look for to find out where is mediation present? Table 5. Randomized total, natural direct and natural...
01 January 1970 744 0 View
I am performing ligation of the plasmid and a target gene. The steps I have taken are: 1. Double digestion of the plasmid and target gene 2. Ligation of the plasmid with the target gene 3....
05 August 2024 2,570 3 View
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05 August 2024 4,407 0 View
I am trying to prepare samples for IP. The pellet size is good and I am using 1% Tx-100 and using a syringe to further lyse the cells and maintain protein-protein interactions. I keep having a...
01 August 2024 7,199 1 View
I am trying to adapt adherent CHO-K1 cells in F12K+10%FBS to suspension culture in only F12K, but in vain. I have followed Mirus bio as well as Thermo protocols, please suggest any alternative....
31 July 2024 3,756 0 View
We have encountered an issue with the serum of mice being hemolyzed after the process of centrifugation. Could you please provide guidance to prevent hemolysis ?
29 July 2024 8,402 4 View
What should be the zeta potential of bovine milk exosomes
25 July 2024 7,376 5 View
through genetic modification or metabolic engineering? Is it helpful, good for health ?
22 July 2024 7,811 4 View
hi, I am facing difficulties in the développement of an ELISA sandwich. I have several experiences in developing ELISA but that’s the first time I can’t find any solution. My test is currently as...
16 July 2024 9,044 10 View
In many experiments where HK-2 cells were stimulated with TGF-β at 10 ng/mL for 48 hours, I observed that even in the control group, the baseline expression level of fibrosis proteins like FN1 and...
15 July 2024 1,929 2 View
Hello everyone. I am working on my LV concentration porject, I use 500Kda hollowfiber to do it. The HCP and BSA removal rate is nearly 80%, but last time, I failed to run it. The HCP hasn't been...
11 July 2024 5,380 0 View