Can anyone tell me which mass technique should be used for polyaromatichydrocarbons (alkyl perylenes)?

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Minimum concentration of protein to be injected for detectable peaks of protein in 16/60 Sephacryl S-100 HR column for SEC-FPLC?

Hello Peers, Warm wishes to all. I am a new user of the Cytiva AKTA Start system for SEC with a column, HiPrep 16/60 Sephacryl S-100 HR (120 ml column volume). I am not sure about the minimum...

03 June 2024 7,713 2 View

How to split molecules into test and training set in 3D-QSAR?

Hi peers and experts! I am new to 3D-QSAR and tried to build a model based on 34 molecules, split into 10 test and 24 training sets. In the end, a poor model is evident from r2 and q2 values....

08 April 2024 2,785 6 View

What is Repeated Earthquakes?

What is the concept of repeated earthquakes? How does the dynamic analysis is performed under repeated earthquake? Is this done in a different way that IDA analysis? How the ground motion data...

02 November 2023 4,884 4 View

While doing simulation on SCAPS-1D for the CdTe/CdS solar cell, I am getting a negative QE for higher wavelength range?

I required some data for the study related to QE of solar cell, but the problem is that I am getting negative QE values at higher wavelength range or at photon energy less than the band gap of...

11 October 2023 5,729 0 View

Does the results obtained with the cracked version of SAP2000 match with licensed version of SAP2000?

I am trying to verify the results obtained by modal analysis and response analysis of a G+4 RC hill Building with the base paper results but unfortunately couldn`t. Although I have followed all...

09 October 2023 7,390 9 View

Why my Ni structure is not converging after applying a strain in X-direction?

Please find the attached Ni Quantum Espresso Input file. &CONTROL calculation = 'scf' verbosity = 'high' restart_mode = 'from_scratch' tstress = .true....

11 September 2023 9,925 0 View

What is the role of disorder in vortex dynamics and vortex lattice melting?

Unconventional superconductivity

30 July 2023 9,168 2 View

How to select the pseudo potential in case multi-component alloys In Quantum Espresso?

I am going to simulate the alloy having three elements in it. I want to know how to select the Pseudo Potential for different alloy elements.

25 July 2023 7,425 1 View

How to calculate the force on each atom after applying the strain on the crystal system in Quantum Espresso?

Here the Ni has equilibrium lattice constant of 3.52 and I applied the 20% strain on x y and z axis. &CONTROL calculation = 'scf' verbosity = 'high' tstress =...

21 July 2023 611 12 View

What is difference between protein expression and activity?

I am finding it hard to understand the difference between protein expression and what is protein activity. Like if my protein is getting expressed in western blot how can I relate it with its...

21 June 2023 1,794 3 View

Which type of compound does lamda max of 218 indicate in a uv-vis spectrum of a partially purified compound through column and TLC?

A crude extract of fungal culture using EtOH was subjected to column and TLC and partially purified compound was obtained. UV vis spectrum of the compound/s has max absorbance at 218nm. The...

11 August 2024 9,801 2 View

Can you connect an HPLC to a Mass Spec only at a certain time point?

Can anyone explain this method? Especially the last statement where it says only at 1.5 to 2.5mins was the MS/MS connected to the UPLC. How is that possible, is it a feature in this specific...

11 August 2024 8,141 3 View

GC-MS retention index prediticon?

Hello experts, Does anyone know any free software about retention index prediction ?

08 August 2024 7,403 2 View

How to calculate CCS for Sodiated adduct ions and Multiply Charged Ions?

I'm currently working on calculating the collision cross section (CCS) for various ions, and I'm facing challenges when dealing with sodiated and multiply charged ions. Most of the resources I’ve...

08 August 2024 8,329 0 View

Can I use a HisTRAP column for affinity chromatography?

I'm working on selecting antibodies against a recombinant protein that has a His-tag. My idea is to first bind the recombinant protein to a HisTRAP column and then use this column for an affinity...

07 August 2024 505 3 View

Weak DAPI staining after immunohistochemistry - how to improve?

After immunohistochemistry of previously fixed in PFA and EtOH and then frozen 20 μm sections of zebrafish brain, DAPI staining is very weak (right) compared to the same sections stained without...

05 August 2024 9,637 2 View

How can major oxides determined by ICP-MS/ES be normalized to 100% volatile free? How does this connect with LOI?

I am analyzing cherts from different sources found within one specific geologic formation using ICP-MS/ES. My committee was asking this question about my analysis. I was not sure how to answer this

05 August 2024 7,902 3 View

Usage of internal standards in LC-MS/MS analysis?

Have you ever seen a LC-MS/MS method uses both internal standards and external standards (in matrix matching purpose) but the concentrations of internal standards are outside the calibration curve...

05 August 2024 3,084 6 View

How to isolate lymphocytes from mouse spleen?

I have tried several times to isolate lymphocytes from mouse spleen, but all attempts have been unsuccessful. I tried most available protocols. I used different dissociation media (HBSS with Ca...

04 August 2024 9,913 7 View

Why is TLC not a form of partition chromatography?

Why is TLC not a form of partition chromatography and paper chromatography not a form of absorption chromatography?

03 August 2024 7,035 3 View