Can anyone tell me what the limitations of using markers are in ecology?

More Atena Eslami's questions See All

Can increasing bacterial concentration in a formulation compensate for inhibition by lactobacillus strain,and if so ,is it right to do so?

I am conducting research on the antimicrobial synergism activity of lactobacillus strain with B.subtilis. in a recent experiment, we observed inhibition of B.subtilis growth with high...

01 June 2024 338 2 View

Can anyone offer advice on how to show the inhibition zone of bacillus against s.aureus?

Hi, i am doing research on the potential antimicrobial activity of Bacillus against s.aureus.i have tried various methods for six months.. like soft agar overlay method but my bacillus colony keep...

26 May 2024 2,151 6 View

Why the sequence of my PCR poduct is very clear without any noise, but very short in size?

I faced with a problem, and I think you can help me regarding this issue. I have sent one of my PCR poducts (ITS1-ITS4) to a company in order to sequence it. Indeed, I sent them various PCR...

01 February 2024 2,626 3 View

Why did I observe more than one band in my PCR product?

What is your solution regarding extra bands in ITS molecular markers? I am trying to use ITS1 and ITS4 markers in 15 plant species. Unfortunately, I only received extra bands instead of one after...

28 November 2023 9,305 4 View

What is PCR bias?

I've recently faced a question that I couldn't solve on my own or with colleagues. I want to know what are PCR biases and how to handle them? Any answer in welcome.

20 June 2023 9,275 1 View

Questions about the bacterial taxonomy ?

Hi all, I have two questions regarding taxonomy 1- how do you define a novel family? or even order? 2- if you have a strain that is not deposited in two culture collection, can you describe it...

20 June 2023 6,362 1 View

Dear professors, would you please let me know in which case it is possible to synonymized taxa?

I have two morphologically similar species that differs molecularly only by 4 bases. Can I synonymmized them? If not, how many base differences can lead to this taxonomic revision? How do I deal...

10 April 2023 3,602 18 View

What is the highest power transferred wirelessly?

Hi All, I was wondering what is the highest power that has been transferred wirelessly in the state-of-the-art research? and what was the furthest distance for it?

11 March 2023 2,735 4 View

MonteCarlo simulations for light tissue ?

What is simple way of explaining Monte Carlo simulations for light tissue? I read some papers, but still need more simpler way of explaining Monte Carlo for light transport in multi-layered tissue.

11 June 2022 5,052 0 View

Measuring displacement in compression testing of masonry prism?

In compression testing of masonry prism, axial displacement can be measured as the relative displacement of the loading platens. What do you think about the associated uncertainties? Is the...

05 January 2022 6,008 5 View

Are there instances where molecules with larger molecular weights exhibit greater mobility than those with smaller molecular weights?

Hi, I know that low molecular weight (MW) molecules generally tend to have higher mobility, while high molecular weight molecules tend to have lower mobility. However, in my experimental...

06 August 2024 1,495 2 View

If we are using snowball sampling technique, how do we justify the true representativeness of the sample statistically? is there any statistical test?

Are there any statistical methods to justify your sampling technique using SPSS or AMOS?

05 August 2024 9,153 4 View

For an in-vitro drug release study, what molecular weight cut-off (MWCO) dialysis bag is required for a 117 kDa protein?

kindly reply me. Thanking you in advance.

05 August 2024 7,727 4 View

What are possible strategies can be used to analyze data under sequential explanatory mixed method approach?

Better ways to analyze the qualitative and quantitative data in a sequential explanatory mixed method approaches

04 August 2024 2,703 6 View

How to start a Molecular Dynamics Simulation?

Is it possible to conduct a molecular dynamics simulation to see the effects of a specific carbohydrate on the structure of lipids (e.g., micelle structure)? I am a beginner in this field and plan...

03 August 2024 3,371 3 View

Which will be the best software for the Hydration shell analysis with molecular dynamics?

I am using a windows system, what software I should use for hydration shell analysis with molecular dynamics?

02 August 2024 3,143 4 View

Can anyone provide me with molecular docking softwares/ websites?

Molecular docking software/ websites?

02 August 2024 8,704 7 View

Why 3 replicates for most biological assays? Is it enough to examine the data fits normal distribution?

Just bounced on me. Before statistically analysing significant difference, shouldn't we see if data fits normal distribution first? Is 3 replicates enough to testify the hypothesis of normal...

31 July 2024 8,141 13 View

Request for Advice: Starch Metabolism Research Project?

I am currently considering a research project focusing on a comparative analysis of starch metabolism in orchids and roses. I am particularly interested in identifying the types and quantities of...

30 July 2024 4,267 2 View

Can the limit of quantification (LOQ) of an analytical method fall outside its linear dynamic range, or must it always be within it?

Can an analytical method's limit of quantification (LOQ) be outside its linear dynamic range, or is it always required to be within it? Please provide a thorough explanation supported by verified...

29 July 2024 7,198 9 View