Can anyone tell me what it's like working with liver FFPE tissues? What are some difficulties you face when slicing and mounting?

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What are some IHC markers that stain the liver structure?

In tonsil we normally use CD3 and CD19 to help stain the overall cross section. If I was looking at a cross section of the liver, what antibodies should I use to help me see the overall...

09 September 2018 7,521 2 View

What are some IHC markers that stain the liver structure?

In tonsil we normally use CD3 and CD19 to help stain the overall cross section. If I was looking at a cross section of the liver, what antibodies should I use to help me see the overall...

08 September 2018 1,484 3 View

Can anyone tell me what it's like working with liver FFPE tissues? What are some difficulties you face when slicing and mounting?

I've worked with human tonsil and brain but never with liver. Thanks!

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Is there a problem with my RNA pellet?

Hello, I am currently having problems with RNA extraction. I am using mouse liver (C57BL6J), and I have extracted RNA from mouse liver before. Before this experiment, my final RNA pellets were...

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Microtome tissue sectioning - How to prevent chatter of paraffin tissue specimen sections?

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What is the best practice for resuspending cell pellets during competent cell preparation (both electro and comp)?

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Tissue homogenization in mouse with FastPrep-24™ 5G - is the D matrix system useful?

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For the process of preparing decellularized matrices what is the ideal solution in which we can bring the tissue from slaughter house to lab?

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Culture fibroblasts with or without FGF2?

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While using the IHC PROFILER plugin in image j software, to quantify the epithelial cytoplasmic DAB staining do you crop remove the stromal tissue?

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Gudrun Lang

It is more like tonsil than like brain. I think if you managed sectioning tonsils, you will also succeed with liver.

Sejal Mistry

Gudrun Lang Thanks! Is there anything I should look out for when I'm doing IHC on them? Is it prone to more auto fluorescence or has high background?

Formalinfixed liver shows autofluorescence and is rich in biotin.

Chris Gilbert

Liver has a tendency to dry out when sectioning, so a wet ice tray helps to prevent them cracking when sectioning. It is very prone to high background, even when using polymers.

What are some IHC markers that stain the liver structure?

What are some IHC markers that stain the liver structure?

Can anyone tell me what it's like working with liver FFPE tissues? What are some difficulties you face when slicing and mounting?

Is there a problem with my RNA pellet?

Microtome tissue sectioning - How to prevent chatter of paraffin tissue specimen sections?

Do you have good tips for seaweed tissue preservation in the field for post RNA extraction?

How to Transform my handy-wavy explanation of why group I/II metals oxides in aqueous solution are basic into a rigorous argument​?

What is the problem with these tissue culture plants?

What is the best practice for resuspending cell pellets during competent cell preparation (both electro and comp)?

Tissue homogenization in mouse with FastPrep-24™ 5G - is the D matrix system useful?

For the process of preparing decellularized matrices what is the ideal solution in which we can bring the tissue from slaughter house to lab?

Culture fibroblasts with or without FGF2?

While using the IHC PROFILER plugin in image j software, to quantify the epithelial cytoplasmic DAB staining do you crop remove the stromal tissue?

How to Transform my handy-wavy explanation of why group I/II metals oxides in aqueous solution are basic into a rigorous argument?