Hi guys. Can anyone tell me the suitable stimulation conditions to stimulate pStat1, pStat3, pStat4, p38 and pAkt. I am working with mouse spleen, bone marrow and murine cell line BaF3. I am optimizing antibodies.
In mouse spleen and bone marrow, IFN-gamma treatment for 30 min. should induce robust pSTAT1 and STAT4 induction, IL-17 treatment results in high levels of pSTAT3, p38 can be induced with TNF. With BaF3 I have no experience.