Can anyone tell me the best and the easiest assay method that can be used to determine the activity of tyrosinase in human Sera or plasma?

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Saeed Abbas

may be helpful:

http://cancerres.aacrjournals.org/content/39/9/3485.full.pdf

http://jcb.rupress.org/content/97/2/480.full.pdf

Jeff Nelson

Indirect ELISA using serum as your coating agent. It may be difficult finding anti-species tyrosinase labeled conjugate.

Hathama Razooki Hasan

thank you Saeed & Jeff for your answers.I have came to a method where trypan blue is used .Any one have any idea about this method