Can anyone tell me Human Hematopoietic Stem Cells doubling time?

More Pawan Kumar Raghav's questions See All

How to add a mass source term in the continuity equation?

Hi, I am trying to model laser cladding in Ansys FLUENT. There are two phase: one is base metal surrounded by an inert gas (shown in the image). I want to add a cladding layer of the same metal....

07 July 2024 1,182 8 View

Electrolyte for electro-polishing D3 steel?

Require the details of the electrolyte used for electropolishing D3 steel.

29 May 2024 1,119 0 View

Please suggest few trending & futuristic PhD Research Topics/ Gaps in Management (preferably Service Quality Management) in Higher Education in India?

Please suggest a few trending and futuristic PhD Research Topics/ Gaps in Management (preferably in Service Quality / Quality Management) in Education (preferably in Higher Education) in India....

08 April 2024 3,664 3 View

Kindly help me how to plot Near Field radiation pattern of an antenna in CST Studio suite?

03 February 2024 3,201 4 View

Anyone can help please how to customize the plots of Boltztrap2 code?

I am performing some thermoelectric calculations using Boltztrap code. The plots I obtained are merely rough plots and I need to customize them , how can i do this?. I am also unable to chose...

25 October 2023 9,121 0 View

How to calculate Dihedral angle analysis of Glucose chain?

Hello researchers, I have processed a MD simulation study of 26 amino acid amylose chain in water using gromacs. Now i want to analyze Dihedral angle for the glucose residues alpha 1-4 linkage,...

18 October 2023 2,003 4 View

Permittivity and conductivity variation with frequency(2-18 GHz) for Aleo vera gel?

Respected Sir's, I have just started working on Microwave Imaging for Food Contamination detection. I need Permittivity and conductivity variation with frequency(2-18 GHz) for Aleo vera gel to...

04 September 2023 4,878 1 View

Recombinant protein expression in HeK cells?

I tried to express a protein in HeK 293T cells cloned in pcDNA 3.1 c myc his, I checked the transfection efficiency by co transfecting gfp vector. It showed good transfection efficiency. But when...

22 May 2023 4,262 4 View

How to start on Research Design and Sampling Techniques in Cyber Security & Cryptography ?

I need to understand what & how to choose sampling techniques I should pick to explore cryptography issues in cyber security domain.

16 May 2023 8,963 5 View

Why titration method is more accurate than HPLC method?

In different Pharmacopoeias monograph, Titration method is still used for assay determination, however HPLC is advance technique.

02 March 2023 4,745 6 View

What are the emerging trends and opportunities for STEM education and careers in the online space?

10 August 2024 507 0 View

Is there any way to quantify bacterial and fungal cells in their mixed culture?

I am working in fungal fermentation of soybean meal and there is bacterial growth in them at times. I am trying to quantify fungal cell counts and bacterial cells; but I haven't been able to do at...

07 August 2024 7,535 4 View

Why activated CAR-Jurkat cell could not kill targets?

Previously when I co-coluture anti-CD19(FMC63) CAR-Jurkat with Raji with E:T=5:1, Jurkat can eliminate Raji in 24h. However, when I test another CAR construct, although I can dectect totally CD69...

06 August 2024 641 2 View

How much total RNA concentration to be extracted from sorted plasma cells from bone marrow of C57BL/6 mice for RT-PCR ?

i have sorted anti-NP specific plasma cells from bone marrow of C57BL/6 mice at certain times after immunization with variable counts and isolated total RNA using TRIZOL method for RT-PCR using...

05 August 2024 8,835 1 View

How to use Density Functional Theory to calculate carrier mobilities of solid system?

Hello, everyone. I have tried to determine carrier motilities of some materials, by Density Functional Theory, using Quantum ESPRESSO. There are a few methods to do it, like a package called...

04 August 2024 8,894 1 View

Does long-term culture of murine Monocytes result in macrophage differentiation?

Hi, I am isolating monocytes from the bone marrow using the Mouse Monocyte EasySep kit. I want to treat these cells and monitor expression of specific markers over the course of 10 days. I will...

04 August 2024 7,282 2 View

How can I get my Granzyme B flow cytometry stain to be consistent?

I have used PE and PE-Dazzle 594 fluorochromes and have managed to get NK cells to properly show GranzymeB expression after 4 hr PMA/ionomycin stimulaton, but for some reason my CD8 cells in the...

01 August 2024 7,677 2 View

Best mask design for metal deposition aiming build a Perovskite solar cell device?

What types of mask designs for metal deposition, to be used in a PVD system, are best suited for perovskite-based solar cells?

31 July 2024 4,835 3 View

How to culture and maintain Lx-2 cells?

Hi there! I recently started working on Lx-2 cells. When I thaw the cells, they start attaching within few hours but later in about 2 days they started rounding and dying. I used DMEM+2% FBS...

31 July 2024 3,893 0 View

How to treat monolayer culture cell line with essential oil?

Hi all, may I ask you for advice about treating monolayer culture cell line with essential oil. Due to the hydrophobic character of the oil, I am concerning how can it equally affect to all the...

31 July 2024 5,301 2 View