Dear all,
I am studying the effect of some physical and biological process on the decontamination of Aspergillus mycotoxins (Ochratoxin A (OTA) and Aflatoxins AFB1). So, i need mycotoxinogenic fungi to artificially contaminate food matrices, particularly wheat, in order to evalute the efficacy of these treatments as decontamination methods.
Best regars,
Amel.
Amel, certainly type cultures of high mycotoxigenic strains are of limited scope and non available in culture collections. However given that there is a high amount of substrate specificity, biotic and abiotic factors that influence the toxigenicity of filamentous fungi, you could try experimentally mimicked scenarios with isolates of Aspergillus ochraceus or Aspergillus flavi species complex i to tranfect and produce OTA and Aflatoxins. I am sure you would be at ease to recover them from cereal plants.
Dear sir,
Thank you very much for your answer.
i agree that it is better to recover them from cereal plants but it needs much work and time to isolate and characterize such strains. The goal of my work is to assess the efficacy of some physical and microbiological methods as mycotoxin decontamination process of contaminated matrices such wheat. So inoculating wheat kernels with OTA or AFB1 fungal producers will be useful to simulate the natural contamination way. i am focusing on the structural and toxicological behaviour of already produced mycotoxins that' s why i need characterised strains with high mycotoxin production rate .
Best regards,
Amel.
Hi , you shoud isolate Aspergillus species like Asp. flavus or Aspergillus parasiticus and Aspergillus nomius its produced aflatoxin and compared with decontamination process.
good luck.
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