27 February 2015 3 6K Report

I have a PCR product sample which was extracted about 1 year ago. I tried to electrophoresis it and a bright band appeared! Then I diluted it 9 times, 4 times and 2 times into 3 tubes and continued electrophoresis but no band appeared!

Can anyone explain why the second time I did electrophoresis there was no band?

Thank you!

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