I have a problem to visualize a crRNA (42nt) alone using the PAGE (1x Tris-Glycine native pH8.5 as a running buffer, 200 V, 35 min). At the same time, tracrRNA (74nt) is seen very well. Just to say, I had no problem with the crRNA detection by PAGE when TBE1x was used as a running buffer. Apparently, Tris-Glycine or/and pH 8.5 impact the crRNA entering the gel.

Do you have any explanation of what happens? Thank you in advance