Can anyone suggest why I cannot see a crRNA band at the native gel (Tris-Glycine pH8.5)?

I have a problem to visualize a crRNA (42nt) alone using the PAGE (1x Tris-Glycine native pH8.5 as a running buffer, 200 V, 35 min). At the same time, tracrRNA (74nt) is seen very well. Just to...

01 October 2020 3,957 0 View