How to detect phosphorylated protein by western blot ?

Dear expertise, 1) I have some trouble to get reproducible results of phosphorylated protein from western blot. Please give me some ideas and suggestions to get the perfect/reproducible results...

15 March 2020 6,690 4 View

Could anyone help me with TEM analysis of my bacteriophages?

26 January 2015 9,759 17 View

Could anyone suggest me a method for purifying phages particles in the SM buffer for TEM analysis ?

I performed TEM analysis for my bacteriohage.repetitively I got only salts images how to avoid the presence of salts during TEM analysis.

24 December 2014 6,470 6 View

Why do my Vibrio species change their nature of yellow color to green color on TCBS plates after 36 hour incubation?

22 May 2014 6,754 8 View

Why did I get a small amount of plasmid DNA during bacterial Genomics DNA extraction/ isolation?

see above

09 March 2014 2,327 6 View

Can someone help with computer-aided drug-design platform using PyMOL?

How to The file Settings_Linux.txt needs to be downloaded and copied to your home directory? reff site: http://people.pharmacy.purdue.edu/~mlill/software/pymol_plugins/install.shtml I have...

08 January 2014 2,149 3 View

How to extract and purify the Bacteriophage particles against pathogenic bacteria? - please suggest me good ideas and research articles

Need suggestion, good ideas and research articles

17 June 2013 6,974 0 View

Could anyone tell me how to calculate the adsorption rate of bacteria and bacteriophage? with any time duration and/or mathematical calculation

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12 June 2013 1,004 3 View

How to prepare adherence cell line from THP-1 suspension cell line ?

Dear experts, I would like to work on THP-1 as adherent cells without changing its natural. Is there any method or protocol for develop adherence cell from suspension cell lines?

01 January 1970 9,871 1 View

Gel electrophoresis result for total RNA samples, 1.5 Agarose gel was used, How to improve the integrity of RNA?

Gel electrophoresis, RNA degradation, RNA extraction from fresh tissue

02 March 2021 5,433 5 View

Problem with shRNA transfection. Why are only two plates getting contaminated instead of all?

I transfected my LNCaP-WT cells with 3 shRNA plus their NTC two weeks ago and split two puromycin selected cell plates on Friday last week(Feb 26). I checked for GFP in the cells, and they all...

28 February 2021 4,949 3 View

Any specific pipeline or parameter to analyse total transcriptomics study of brain tissue samples?

I have two groups of brain samples, control and treated for example. It was total RNA nova seq sequencing. I tried all the available pipeline like: star+rsem+deseq2, Hista+stringtie+cuffdiff,...

27 February 2021 356 6 View

Why is the efficiency of viral mRNA electroporation in PBMC low?

Hello, I have been struggling with the electroporation of PBMC with HIV-I mRNA. Although the quality of the RNA generated is good, and the handling of the cells in meticulous, the frequency of...

27 February 2021 9,233 3 View

LEDs on Vis Light photo degradation of MB water sol via N doped TiO2 in a mini flume?

Good day to all, I have no experience with light emitting diodes. I am building a glass mini flume to demonstrate the photo degradation of Methylene Blue in water running over self cleaning...

26 February 2021 4,026 5 View

What is the best concentration of cDNA to be used for qPCR?

I have synthesised cDNA from 500ng/ul of RNA. The concentration of the cDNA Is about 5000ng/ul. Should I dilute my cDNA or just use it as it is for my qPCR? I usually use 2ul of cDNA for a 20ul...

24 February 2021 8,618 3 View

Recommendations on homogenizers for RNA isolation?

I will be performing RNA isolation with frozen lung biopsy samples and am in the market for a tissue homogenizer. I am wanting a homogenizer that will fit in a 1.5ml tube. I was looking at the...

24 February 2021 3,623 3 View

RNA-seq DEGs downstream analysis and reporting in literature?

I used EdgeR to get the list of DEGs (differentially expressed genes) from my liver RNA-seq experiment. I have done the gene overrepresentation and GSEA based pathway analysis. I am currently...

23 February 2021 8,969 1 View

Is it efficient to do liquid biopsy to investigate the gene expressions of the tumor?

To do liquid biopsy and look for specific gene expressions there are so many published papers. Thera are different protocols and procedures used to isolate circulating cell free RNA or mRNA from...

23 February 2021 7,326 5 View

RNA degraded after good nanodrop readings?

23 February 2021 8,850 2 View