We have A thaliana seeds and tried to grow them in normal culturing agar medium and soil. It is now 6 days and we did not get any growth.
It is weird, because germination breaks after 3-4 days after sowing. What are your growing conditions?
we kept three different conditions
1. in mineral salt medium at 25C
2. Mineral salt medium in room temperature (30 C)
3. In soil at room temperature
seeds were socked in the water due to moisture developed in the plate.
Not sure, if your procedure includes the vernaliztion step.
Seeds are kept in water or on agar in the fridge or cold room for 1-2 days in order to break dormancy. You then proceed with sowing on soil or growing in a phytochamber as you already did.
Thanks Elisa for your suggestions. We will keep the seeds at 22 ºC and hope for the best.
Thanks Martha
We have followed the cold shock step before sowing the seeds.
Hi Bipinraj,
If the seed is very old, it may not germinate properly. If you are still having problems after trying the two solutions below, I'd say that's your problem.
Otherwise, as others have suggested, it's best to stratify the seeds by placing them in water or on agar plates for 3-4 days at 4oC in the dark.
Also, if growing on soil, it is best to make sure the doesn't dry out or the seeds will not germinate happily. To maintain humidity, try covering the soil with some cling wrap.
Good luck!
Hi Bipinraj,
Germinating a new species in the lab is always a challenge, do not worry you will get there.
in order to properly assist you, I will need to know:
1.Are your seeds Wt? (some mutants need extra nutrients to start the germination process)
If yes, what is the ecotype? (the optimal growing condition are different)
2.In your growth medium are you using bactoagar, tecnical agar or phytogel(sigma)?
3.Are you using MS (Murashige and Skoog)growth media? without sucrose? (sucrose may inhibit germination in some ecotypes)
please take a look at NASC (The European Arabidopsis Stock Centre) recommendations for Arabidopsis culture Ir:
http://arabidopsis.info/InfoPages?template=newgrow;web_section=arabidopsis
Best of luck.
Mário
http://arabidopsis.info/InfoPages?template=newgrow;web_section=arabidopsis