Can crude enzyme be used for immobiliazation?

Can crude enzyme be utilized as immobilized enzyme for industrial application if the crude enzyme shows high activity?

13 July 2020 8,672 4 View

From where can I get microglia cell line like N9 or BV-2 in India ( preferably in Kolkata)?

From where can I get microglia cell line like N9 or BV-2 in India ( preferably in Kolkata)?

05 November 2019 7,719 2 View

How to conjugate a dye to the N-terminus of poly-glutamic acid peptide?

Hi! I am trying to conjugate a dye in its NHS ester form to the N-terminus of poly-glutamic acid peptide ( which is already available in the form of a white solid). I tried 3 times and am tring...

27 June 2019 8,798 3 View

Is it necessary to purify PCR products before cycle sequencing?

I will purify the cycle sequencing products using EDTA/EtOH precipitation method before loading to 96 well plate.

30 November 2018 6,932 4 View

In case of Box behnken design reduced quartic model, do I need to mention all the insignificant terms in the ANNOVA table and polynomial equation?

When I try to reduce those terms the R2 value reduces. As a result I have many terms whose sum of squares and df values are 0. The p value and all other values fall in range.

22 May 2018 8,088 4 View

How to measure voltage dependent strain of a thin film without using electric field dependent XRD???

because we have not any facility of electric field dependent XRD but all the papers suggested this electric field dependent XRD. If anyone can explore any idea then please help me

15 April 2018 263 2 View

In an XRD data if the peak are same with ICSD data but intensity is not matched with ICSD data then is anything worng???

My XRD data for NdNiO3 is similar with ICSD data but intensity are not matching with ICSD data

11 March 2018 8,823 4 View

How a core flood experiment for conformance control can be designed?

The limitation we are currently having is that cores of length upto 6 inch can only be used in our system and we can not prepare composite cores.

03 September 2017 9,093 2 View

Can zero field cooled MH loop measured after some times (few months) for same magnetic sample at same temperature be different?

17 February 2017 6,694 5 View

PLD Vs RF sputtering?

How PLD and RF sputtering are different over each other ? Or why one should choose PLD over sputtering except depositing complicated oxides (e.g: super conductors or perovskites). It is well known...

12 October 2016 1,464 4 View

What are the values needed for gabor filter's parameter for cifar10 data?

Hi, I implemented a code to gabor filter cifar10 data but the images after being filtered and stacked are not clear like the original images. I think the problem is in the way I am using the...

03 March 2021 6,317 1 View

How to calculate Total Flavonoid?

I analyzed flavonoid total spectrophotometry, the procedure as below: 2 ml supernatan added 5 ml dd.H2O, then added 0,15 ml NaNO2 5%, 5 minutes later added 0,15 ml AlCl3 10%, After 6 minutes,...

01 March 2021 6,722 1 View

RNA-seq DEGs downstream analysis and reporting in literature?

I used EdgeR to get the list of DEGs (differentially expressed genes) from my liver RNA-seq experiment. I have done the gene overrepresentation and GSEA based pathway analysis. I am currently...

23 February 2021 8,969 1 View

What are the ranges of four diffterent poles of butterworth filter for ECG signal?

Hi, Can anyone please tell me what are the ranges of four different pole orders such as first order, second order, third order and fourth order respectively of butterworth filter for ECG...

23 February 2021 2,554 3 View

Which polarizers are usually used to measure the birefringence of zebrafish muscles?

I already found several publications on that topic, however, the specifications for the used filters are often missing. Some more specific information than "fish is placed between to polarizing...

23 February 2021 1,934 1 View

What low-cut filter (high Pass frequency) to be used for response spectrum (Tripartite plots) ?

What low-cut filter (high Pass frequency) to be used for the response spectrum (Tripartite plots)? I am currently working on differentiating between hard/soft site responses in Indo-Gangetic...

21 February 2021 9,273 2 View

How can I retrieve FASTA sequence from gene ID or UID of a Gene ? I have GeneBank IDs ?

I treid to use Entrez Batch, but it redirects me to NCBI site. Is there any script of Linux or Python through which I can download Fasta sequence of list of gene ID ?

21 February 2021 7,783 12 View

Is it possible, for a limited dateset of images, to use Convolution filters + Random Forest instead of UNet?

UNet is 'relatively' recent and was invented mainly to deal with biomedical images, yet is it also possible, for a limited dateset of images, to use Convolution filters + Random Forest instead of...

21 February 2021 4,858 3 View

The type of filter paper used to filter an epoxidized oil (the mixture is an epoxidized oil with magnesium sulfate) ?

I have a mixture of epoxidized oil and magnesium sulfate wich type of filtre paper should i use

21 February 2021 3,944 3 View

Blast analysis of RC,UHPC slabs using material model *MAT_72R3 in LS-DYNA?

20 February 2021 5,984 3 View