Can we use RNA-seq library for gene expression studies?

Since our RNA is less abundant, can we use the libraries prepared for RNA-seq to validate (qPCR)the results of RNA-seq.

12 November 2020 803 6 View

For primer efficiency calculation can we use positive control(in which gene is abundantly expressed) and perform the analysis?

My sample of interest is sperm RNA(less abundant and highly fragmented RNA). In that case, to determine the primer efficiency, can i use tissue samples (in which the gene is abundantly expressed)...

11 November 2020 758 3 View

What is the procedure of isolating tissue from canine cancer for performing Proteomics and NGS?

we intend to perform the proteomics analysis and NGS of the isolated tissue from canine cancer model and need the protocol for proteomics and NGS of canine cancer model.

06 October 2019 3,516 3 View

What is the difference between semantic role labelling and named entity recognition?

Natural language understanding

04 May 2019 7,442 9 View

How to calculate network performance using ns3?

I'm new to this network domain. But i want to learn this area thoroughly. So I'm currently dealing this subject. I'm learning ns-3 simulator. Please help me to know how to analyze the performance...

17 September 2018 3,246 3 View

Is it possible to extract RNA from commercial rabies vaccine?

I have tried extraction of RNA from various commercial rabies vaccine which currently used for pre exposure and post exposure for dogs. After that I have done RT- PCR, while running gel i got band...

01 August 2016 1,675 5 View

In XRD peak, when ever doping materials the peaks will be shifted left to right (or) right to left why?

Ex, Sr doped La2CuO4 in XRD peaks will be shifted right to left.

10 February 2016 5,348 2 View

Acyliminium reactivity versus enecarbamate?

Dear researchers, i kindly ask you to help me with the following aspect: in scheme 4 (of j.org. chem, vol.66,No.23, 2001) it is shown that nucleophile attacks on the acyliminium intermediate and...

08 February 2016 1,601 3 View

I am using HEC RAS to connect various ponds and lakes in the city and perform flood inundation mapping. What is the suitable analysis?

I am using HEC RAS (Though it is a river analysis software) to an urban area to connect various ponds and lakes in the city and perform flood inundation mapping. What is the suitable...

01 February 2016 4,321 6 View

Why does K estimator fail to estimate Ka and Ks values for certain gene combinations?

12 February 2015 8,709 1 View

No title

02 March 2021 3,060 3 View

What do you know about biotechnology art?

What do you know about biotechnology art?

02 March 2021 1,653 3 View

Which is the best place in the world for the plant biotechnology research?

I am searching for a good place for the Post Doc,

02 March 2021 4,053 3 View

Is CD44 degraded in lysosome together with hyaluronan after CD44 mediated endocytosis of hyaluronic acid?

Also when RHAMM binds hyaluronic acid, they get internalized, will RHAMM also be degraded? Or both CD44 and RHAMM will be transferred back to the cell membrane? Asking for breast cancer cell line...

01 March 2021 8,169 2 View

Where to find data on trees in tropical agroforestry?

Hi, I'm looking for data (mainly related to management: growth rate, canopy size, soil and climate preferences, etc.) about tropical trees used in tropical agroforestry. Have you ever heard about...

28 February 2021 7,356 8 View

How to compare 2 samples recruited with different sampling methods?

If I want to compare characteristics of 2 groups of patients, one group is recruited from a random sample of the general public while another group is from a sample with a fixed gender (male to...

26 February 2021 3,526 3 View

How was your experience with Biomedical and Biotechnology Journal?

How was your publication experience with BBRJ in terms of peer review and reviewer comments. Did you find it fair and transparent ?

23 February 2021 3,810 6 View

I have a problem with kanamycin - why the kanamycin doesn't affect?

Hi everyone, I need to expression of GFP with pET28 plasmid in E. coli BL21(DE3) plysE. Transformed cells have kanamycin and chloramphenicol resistance. Normally, only transformed cells should...

22 February 2021 4,032 4 View

Do you consider undergoing a structure literature review / Meta analysis and getting numerical findings sort of quantitative method?

I am looking for a classification of the research methods included in literature review papers or Meta analysis ones .

22 February 2021 1,131 4 View

Can I do two kinds of mixed method in explanatory research?

Dear distinguish professors and scholars, I have a research in measuring two kinds: think tank capacity and research impact on policy. Can I do two kinds of mixed methods in order to find, "Is...

20 February 2021 6,897 1 View