Panel of serum or plasma with antibodies to known pathogens?

Does anyone know of a panel of serum/plasma with antibodies to known pathogens such as Influenza A, HBV, HCB etc, that could be purchased for testing? or through ethics application to biobank?

03 September 2020 3,017 1 View

What is the role of the copper precursor in chemical nanoparticle synthesis?

I have been trying to obtain copper nanoparticles in aqueous colloidal suspensions following procedures found in the literature, only I am using copper sulfate instead of copper chloride as Cu2+...

25 January 2018 8,627 3 View

Has anyone tried to increase the Tm of a forward primer with additional random primers at the 3' end? I'm trying with 6N, but it's not working.

I'm trying a gradient PCR to check the best T, previously I have been using 62 as the best option. I would like to increase it to get closer to the Reverse primer, that is 71 degrees. I am using...

13 December 2012 9,012 15 View

Is there a format that I follow to introduce an incubation idea?

I am very new to this project but very excited about collaboration in a global context. Is there a particular format you use that I can prepare a presentation on Creativity? Patricia

01 January 1970 421 3 View

Is there any test to determine the cardiovascular risk of patients with obesity? from the perspective of obesity?

We found tests to determine cardiovascular risk taking into account lipid profile, blood pressure, age, history of diabetes, other laboratories. But when weight is involved, there is a paradox. I...

02 March 2021 6,036 1 View

How can we calculate the sample size for rodents for a particular period of time?

How can we calculate the sample size for rodents for a study for one year? Are there any specific protocols to be followed for designing rodent sampling? How long should be the sampling interval...

01 March 2021 1,056 3 View

Does anyone have a recommendation for yeast reporter gene plasmid/protocol?

Hi, could anyone recommend a plasmid and/or protocol for reporter gene assay in S. cerevisiae? I want to assess the effect of growth conditions on a transcription factor, so I want to clone it`s...

01 March 2021 210 1 View

Would fecal samples stored at -20C for over a year impact metagenomic analysis?

Dear All, I have access to some rodent fecal samples that have been stored at -20C instead of -80C. Anyone have experience with the impact of stowage condition on comparative metagenomic analysis...

01 March 2021 2,512 1 View

What are the pros and cons for mice versus rats?

Hi Everyone, I am designing a pilot study using rodents to assess the effects of simulated space environments (radiation + altered gravity) on microbiome and immune system. I have limited...

24 February 2021 1,825 3 View

Is the box plot of climatic indices better illustrate from daily data or annual data?

Hello, I hope you have a good time. I work on a research project about temperature indices. Due to the high number of indices, I only work on tables and maps on an annual time scale. In other...

19 February 2021 9,742 2 View

What is recommended and opened real-time or nearly real time geospatial data used in flood risk assessment and regional planning on a detailed scale?

I asked recommended real-time or nearly real-time data source for any parameters for flood risk on a microscale analysis for urban area cases in developing countries that had agglomeration urban...

18 February 2021 2,617 2 View

What are backpropagation formulas for residual neural networks?

14 February 2021 4,194 3 View

What is the correct common name for Hydrochoerus hydrochaeris?

There are two extant species of Hydrochoerus: Hydrochoerus isthmius, the lesser or Panamanian capybara, and the genotype species H. hydrochaeris. The latter is the more common species of capybara,...

11 February 2021 3,494 14 View

Has anyone tried eBioscience Foxp3 / Transcription Factor Staining Buffer Set with TdTomato+ cells?

Hi all, I stained tdTomato+ cells (PBMCs and Jurkats) with eBioscience Foxp3 / Transcription Factor Staining Buffer Set for the foxp3 and I completely lost the signal of tdTomato. I tried to fix...

10 February 2021 5,863 2 View