I have preformed the CD spectrum of my protein (Mol. wt. 49 kDa) alone or in the presence of a ligand. I want to analyze the changes happening in the protein secondary structure upon ligand binding. Specifically, I am looking for the % changes in the helics and B-sheet content of my protein in the absence and presence of the ligand. Can anyone suggest any online reliable softwares to do this kind of comparative analysis. I tried analyzing the data with some freewares like K2D2 and K2D3 but the results were not consistent.
Thanks in advance..
Mr. Darpan,
You can use web based tool CAPITO for analysis and plotting your data as given in the link below along with its Publication to understand the tool.
Hope this will help.
http://capito.nmr.fli-leibniz.de
https://academic.oup.com/bioinformatics/article/29/14/1750/231861/CAPITO-a-web-server-based-analysis-and-plotting
Dichroweb is doing good for me
http://dichroweb.cryst.bbk.ac.uk/html/home.shtml
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