Darpan Raghav

3 Questions 7 Answers 0 Followers

Questions related from Darpan Raghav

Calculating the binding constants for a ligand having 4 binding sites on a protein using the Adair equation?

I am working on determining the binding site of a ligand on bovine serum albumin (BSA). From Job's plot and Scatchard plot analysis I could calculate that the ligand has 4 binding sites on BSA....

07 July 2019 7,502 4 View

Three Dimensional Fluorescence Spectroscopy not giving expected results?

Hi, I wanted to study the conformation changes in BSA using Three dimensional fluorescence spectroscopic analysis in a spectrofluorometer. I am using BSA (10 uM) for this analysis and my...

02 February 2018 3,398 4 View

Can anyone suggest me some good online/academic lisence softwares to analyze and interpret the CD spectra of my desired protein?

I have preformed the CD spectrum of my protein (Mol. wt. 49 kDa) alone or in the presence of a ligand. I want to analyze the changes happening in the protein secondary structure upon ligand...

04 April 2017 7,958 3 View