I stained the cells with DAPI after treated with chemicals and then checked with fluorescence microscrope. I would like to know what is the morphology of these cells.
Hello. DAPI is a fluorescent stain that binds strongly to adenine–thymine rich regions in DNA. It is used extensively in fluorescence microscopy. As DAPI can pass through an intact cell membrane, it can be used to stain dsDNA both live and fixed cells, though it passes through the membrane less efficiently in live cells and therefore the effectiveness of the stain is lower.
What do you mean by morphology? What characteristics are you looking at? Many things like size, shape, arrangement margin etc. Did you stain the membranes or nuclei as Sebastian Schmitt
said? DAPI staining method is broadly used for the detection of polyphosphate granules in microbial cells. what kind of cells are you talking about? Please clarify.
Hello. DAPI is a fluorescent stain that binds strongly to adenine–thymine rich regions in DNA. It is used extensively in fluorescence microscopy. As DAPI can pass through an intact cell membrane, it can be used to stain dsDNA both live and fixed cells, though it passes through the membrane less efficiently in live cells and therefore the effectiveness of the stain is lower.
Thank you for your kind suggestion. I used AGS cells to check apoptotic cells by using DAPI staining. But in my case, I used two chemicals. The images obtained from these treatments after DAPI staining were different, so I would like to know what is the problem. I would like to check apoptotic cells and also necrotic ones.
DAPI stain won't identify apoptotic cells, it is only a nuclei staining, identify nuclei of all cells. To identify apoptotic cells, use TUNEL stain or Caspase-3 IHC stain.
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