I just have no patience for entering in contact with the company to ask the prices... I just want to know if there's a good and really cheap kit for detection of mycoplasma contamination...
Sigma has quite a cheap one: http://www.sigmaaldrich.com/content/dam/sigma-aldrich/docs/Sigma/Bulletin/mp0035bul.pdf
Hi Telles,
Most of the kit that you are looking for is based on mycoplama detection by PCR based methods. You can very well synthesize primer pairs (based on many literature available in pubmed) and can detect as low as 1-2 copies of mycoplasma DNA without any cross reactivity to humans or other microorganisms (Pls refer to PMID: 16554716)
No one can really help you here - your university will have different prices than the list prices because it is an academic institution. Other universities may have different conditions, so prices other researchers tell you may not be the ones you will get. Excuse me for being blunt, but if you really want to find out which is the cheapest kit for you, it seems you will have to stretch your patience and make a few phone calls...
The solution involving the least work would be to find a group at your university that already does mycoplasma testing and ask them what they use. Maybe you can even convince them to give you a few tests for free or include some of your samples when they do their next mycoplama test ;-)
People, thank you all for your answers!
I remembered my old lab has a confocal and a fluorescence microscopes. I'm thinking maybe I can use DAPI in my cells and observe them through microscopy to detect mycoplasma DNA in locations other than the nucleus...
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