I am new to using immunofluorescence techniques and confocal microscopy. Can anyone kindly introduce a good reference to me which I can read and understand how to analyze immunofluorescence images, e.g. how to rule out background staining.
Thank you very much Disha. In fact, still I have not faced any problems yet but I'm somejow suspicious to the images even yhr positive stainings that I get. So I decided to find a good reference. If its ok, I'll come to you in case I faced any problems :-)