Hi I think this paper would be helpful to you

http://eprintsprod.nuim.ie/185/1/nematoda_2.pdf

Reproductive compatibility of different species can be conducted

using the method reported by Nguyen and Duncan (2002) using G. mellonella haemolymph. A drop of haemolymph of G. mellonella

is placed in a sterile Petri dish ( 35 × 10 mm) and a single I J of nematode

species A and one IJ of nematode species B are added. Control crosses

are done between IJ of the s ame s pecies. A ll treatments are replicated

30 times. A ll Petri d ishes a re ke pt in closed plastic bags containing

2-3 layers of m oistened facial tissue to prevent the haemolymph from

drying. After 3 -5 days, a single m ale and single f emale w ill deve lop in a

proportion o f the dishes. O bserve them regularly to see if they m ate and

produce off spring. (According to ENTOMOPATHOGENIC NEMATODES:

SYSTEMAT ICS, PHYLOGENY AND BACTERIAL SYMBIONTS; chapter 3; Nguyen and  Hunt, 2007)

Dear Esmaeil Miraeiz ,

We have done this procedure. When a drop of haemolymph of G. mellonella

was placed in a sterile Petri dish and  IJ from two isolates were added and observing them after two to three days nothing was visible as the whole of drop turned black due to melanisation. In that condition what should be done so that we could be able to observe whether cross hybization has occured or not and thanks sir for your knid response