I want to know some efficient method for HPLC anysis of IAA which includes Solvent system, mobile phase, approx elution time etc.
If by IAA you mean, indole acetic acid, then that should be a very easy HPLC method to run. Select a good quality C18 column (e.g. 4.6 x 250 x 5u) and run a gradient of acidified Water (e.g. 0.1 % acetic) vs MeOH at 1.00 ml/min. Use a DAD to scan and detect (max ~ 249 to 254nm) and aim for a K prime of around >3.
If you would try a keyword search on the web, I am sure you could find dozens of examples to review and should have no trouble running this one.
If by IAA you mean, indole acetic acid, then that should be a very easy HPLC method to run. Select a good quality C18 column (e.g. 4.6 x 250 x 5u) and run a gradient of acidified Water (e.g. 0.1 % acetic) vs MeOH at 1.00 ml/min. Use a DAD to scan and detect (max ~ 249 to 254nm) and aim for a K prime of around >3.
If you would try a keyword search on the web, I am sure you could find dozens of examples to review and should have no trouble running this one.
You can use and adapt this method.
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC3568474/
Yes, I agree with Bill, you can find many HPLC method for IAA, just searach via Google or other data bases, best regards
please find link below http://www.ncbi.nlm.nih.gov/pubmed/23111785
If you mean indole acetic acid, you can simply use HPLC. So I agree with Bill Letter explaination. an article is attach to help your research.
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