Can anyone suggest cytotoxic dose of E.coli LPS to THP 1 cells within 24 hrs while doing an MTT assay?

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David G Spiller

LPS activity varies greatly between batches and suppliers. Try using Lipid A instead.

Shen-An Hwang

Quick question... Are you differentitating the THP-1 into macrophages or are you using them as monocytes?

Prajitha Nadukkandy

Thank you David G Spiller and Shen-An Hwang

I am using THP 1 as monocytes@ Shen-An Hwang

Then that's probably why. Macrophages are more sensitive to the toxic effects of LPS.

Thank you Shen-An Hwang for your valuable answers.