Can anyone suggest me commercially available basic luciferase vectors that work in plant protoplasts? Will pGL vectors from Promega work in plant protoplasts as well?
pCAMBIA vectors are equally efficient in plant transformation. Please log on to 'http://www.markergene.com/pcambia-vectors' and made an email you will able acquire solution.
This paper will be useful for you."Firefly Luciferase Complementation Imaging Assay for Protein-Protein Interactions in Plants"
@ Dr Anita Kumari,
That is interesting. I know Cambia Lab is no longer distributing pCambia vectors due to lack of public funding support (according to its website). Now I know that the Marker Gene Ltd is selling them (and the exclusive supplier in the US). Thanks for the info.
Hi Yadukrishnan,
I think that pGL (pGL3, pGL4,...) vectors from Promega are aiming for mammalian cells. Especially the pGL4 vector, where the luc gene (they call it luc2) is especially codon-optimized for mammalian cells. See attached article, pages 9 and 14 (yellow highlights).
Thanks, Dr. Yuan-Yeu Yau and Dr. Anita Kumari.
I had noticed that in the protocol. But wanted to confirm it since I couldn't find any commercial luciferase vectors for plant protoplasts.
I could neither find any pCAMBIA vectors with luciferase in it. I think in most of the articles modified vectors made of plant vector (such as pCAMBIA) backbones edited and inserted with luciferase are being used. Moreover, what I wanted to study is not the protein-protein interaction but the expression regulation of one gene by a transcription factor. What I am looking for is a commercial vector in which I can insert the promoter of my gene of interest and study how the TF regulates its expression.
Thank you.
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