Can anyone suggest any manual method of cDNA Synthesis?

16 March 2022 1 2K Report

I am new to RT PCR method. I have purchased all the cDNA Synthesis reagents separately, but not any kit. I did not find any protocol or paper regarding manual cDNA Synthesis. Can anyone please help me with this?

Thank you in advance

Sreyasi!

Muhammad Bilawal Arain

follow the below paper

https://www.affymetrix.com/support/technical/other/cdna_protocol_manual.pdf

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