14 Questions 10 Answers 0 Followers
Questions related from Sreyasi Das
Hello all! I am analyzing adipocyte areas in human subcutaneous adipose tissue sections. I have scanned whole-slide images, which are in SVS file format. I am extracting portions of these images...
04 July 2025 6,501 0 View
Hello everyone! I did T cell immunophenotyping in murine splenocytes. I want to do tSNE analysis of different transcription factors in Th and Tc subsets. I am analysing my data in latest version...
05 December 2022 5,169 0 View
I am doing routine hematoxylin and eosin staining of mouse spleen. I found frequent round fibrous structure marked red in the picture. can anyone guide me to identify these? Thanks in...
16 August 2022 2,485 2 View
hello!! I need to calculate glomerular Feret's diameter in mice kidney sections with imagej software. Can anyone help me with the protocol? Thank you in advance!
15 July 2022 5,119 1 View
Hello all!! I am currently quantifying damage in renal histological sections of AKI model. I found EGTI scoring system during the literature search. However, I did not find the detailed protocol...
26 June 2022 6,451 1 View
I am new to RT PCR method. I have purchased all the cDNA Synthesis reagents separately, but not any kit. I did not find any protocol or paper regarding manual cDNA Synthesis. Can anyone please...
16 March 2022 2,512 1 View
Hello! I want to isolate kidney infiltrating mononuclear cells for flow cytometric analysis. But I don't have enough collagenase 1 for digestion. Is there any method exist by which I can isolate...
09 February 2022 2,233 0 View
I have to quantify a mixture of proteins. But I can not quantify its concentration by any protein detection methods as the values fluctuate. Can i quantify the protein concentration by sds page?
23 February 2021 659 2 View
I am characterizing different snake venoms. As snake venom is a mixture of various large protein complexes (with both acidic and basic proteins) i want to run native agarose gel electrophoresis...
08 December 2020 4,868 3 View
we are doing flow cytometric analysis of mouse T lymphocytes. We want to check the nuclear transcription factors. Currently we are running low on PMA and ionomycin. Is it possible to do the...
07 October 2020 2,973 3 View
Hello! I want to measure GFR in mice but currently don't have FITC or radiolabelled inulin. Can i measure GFR with untagged inulin? Thanks in advance Sreyasi.
15 September 2019 968 1 View
I want to collect 18 hr fasting urine in mice. For my study, i have to bring all the animals at same water level (i am working in a nephrotoxic Acute Kidney Injury model). What is the proper time...
10 May 2019 1,001 0 View
Hello all, I want to isolate kidney infiltrating lymphocytes using enzymatic digestion. I have found a protocol with type 1 collagenase (10mg/ml concentration). can i use trypsin as an alternative...
19 February 2019 828 2 View
Hello!! I want to do gelatin zymography for MMP-9 and 2 in mice urine but overnight urine collection is not suitable for enzymatic analysis as they can degrade. so i want to collect urine...
02 September 2018 2,327 4 View
