Sreyasi Das

10 Questions 8 Answers 0 Followers

Questions related from Sreyasi Das

Is it okay if cell populations change their position in tSNE plot?

Hello everyone! I did T cell immunophenotyping in murine splenocytes. I want to do tSNE analysis of different transcription factors in Th and Tc subsets. I am analysing my data in latest version...

05 December 2022 5,110 0 View

What are these fibrous structures in mouse spleen?

I am doing routine hematoxylin and eosin staining of mouse spleen. I found frequent round fibrous structure marked red in the picture. can anyone guide me to identify these? Thanks in...

16 August 2022 2,430 2 View

How to calculate Feret's diameter of mice glomerulus?

hello!! I need to calculate glomerular Feret's diameter in mice kidney sections with imagej software. Can anyone help me with the protocol? Thank you in advance!

15 July 2022 5,052 1 View

Can anyone suggest any manual method of cDNA Synthesis?

I am new to RT PCR method. I have purchased all the cDNA Synthesis reagents separately, but not any kit. I did not find any protocol or paper regarding manual cDNA Synthesis. Can anyone please...

16 March 2022 2,441 1 View

Can i quantify protein concentration in sds page?

I have to quantify a mixture of proteins. But I can not quantify its concentration by any protein detection methods as the values fluctuate. Can i quantify the protein concentration by sds page?

23 February 2021 456 2 View

Can i run native agarose gel electrophoresis in TAE buffer?

I am characterizing different snake venoms. As snake venom is a mixture of various large protein complexes (with both acidic and basic proteins) i want to run native agarose gel electrophoresis...

08 December 2020 4,789 3 View

Is it possible to do flow cytometric analysis of transcription factors of T lymphocytes without any PMA, ionomycin stimulation?

we are doing flow cytometric analysis of mouse T lymphocytes. We want to check the nuclear transcription factors. Currently we are running low on PMA and ionomycin. Is it possible to do the...

07 October 2020 2,911 3 View

Can i measure GFR in mice with single intravenous injection of untagged inulin?

Hello! I want to measure GFR in mice but currently don't have FITC or radiolabelled inulin. Can i measure GFR with untagged inulin? Thanks in advance Sreyasi.

15 September 2019 918 1 View

What is the most suitable technique for overnight urine collection for mouse?

I want to collect 18 hr fasting urine in mice. For my study, i have to bring all the animals at same water level (i am working in a nephrotoxic Acute Kidney Injury model). What is the proper time...

10 May 2019 951 0 View

Can i use trypsin in place of type 1 collagenase in mouse kidney digestion process?

Hello all, I want to isolate kidney infiltrating lymphocytes using enzymatic digestion. I have found a protocol with type 1 collagenase (10mg/ml concentration). can i use trypsin as an alternative...

19 February 2019 770 2 View