I am trying to extract RNA, from grape berries of a red variety, for qPCR (to study gene expression). I have tried nucleozol and a CTAB protocol (with b-mercaptoethanol+boric acid + cleaning with chloroform, but without success (Actually after cleaning with chloroform and transferring the supernatant, I added isopropanol to the supernatant and got 2 liquid fractions instead of a pellet (after centrifuge)). Do you have an idea why that happens? Can someone please suggest a protocol?
Hello Danai,
I found these 3 protocols. Might be helpful.
https://bio-protocol.org/e1777
https://www.sciencedirect.com/science/article/pii/S0254629912001214
Article High quality RNA extraction from Maqui berry for its applica...
Regards,
Giannis
Hi Danai
You can find a method in this paper
" A new and simple method for rapid extraction and isolation of high‐quality RNA from grape (Vitis vinifera) berries "
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