I need to isolate DNA and further use it for PCR amplification purposes.
Does the ratio between mitochondrial fission vs fusion indicate mitochondrial biogenesis or is their a specific marker to anaylze mitochondrial biogenesis reliably? Looking forward to...
05 June 2018 4,100 4 View
I am trying to set up different assays to measure levels of reactive oxygen species. I would appreciate if anyone could share their experience regarding the usage of Dihydrorhodamine 123 to detect...
07 August 2017 7,704 3 View
I have two images of the same sample with different x,y,z dimensions. Can anyone explain - in order to quantify the same object - why do I need to enter larger number of voxels in the size filter...
05 June 2014 8,253 9 View
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08 September 2013 2,100 1 View
Even after specifying the flexible residues in the ligand file when I go ahead to save it as flexible pdbqt the prog says- " Must Specify Residues for flexible side chains or set hinge". Can...
03 April 2013 4,883 8 View
I am unable to find the ata related to the binding sites of my chosen protein. I have tried using Q Site Finder but its not helping much. Can anyone please suggest some other free online tools...
02 March 2013 2,069 15 View
I'm cloning a fragment of 3200 nts into plasmid. The cloning was successful, however, 02 amino acids were mutated. Now I want to fix these 02 aa by site-directed mutagenesis technique using...
08 August 2024 4,645 2 View
After performing symmetric PCR, PCR purification was performed. Afterwards, asymmetric PCR was performed using the PCR purification product as a template, but no ssDNA band was confirmed in the...
08 August 2024 1,668 3 View
I'm trying to find a DNA extraction method for fungi that does not require equipment and heating. Is there anyone who can suggest an alternative option? Thank you
08 August 2024 4,733 2 View
i have sorted anti-NP specific plasma cells from bone marrow of C57BL/6 mice at certain times after immunization with variable counts and isolated total RNA using TRIZOL method for RT-PCR using...
05 August 2024 8,835 1 View
Now research fellow used it to prevent fungus in potato,may be potatoes gene effect.
02 August 2024 180 3 View
I have been attempting to extract DNA from Bacterial, Fungal and Yeast banked samples (>1e7 cells) using Prepman Ultra reagent and I seem to be struggling to obtain a sequence. Although the...
01 August 2024 2,079 0 View
Please discuss someone told about fungus and allied others.
31 July 2024 3,372 3 View
Hello everyone, I performed a PCR yesterday, and the results showed no bands on the gel. Of course, I probably missed some crucial steps, like adding my samples to the PCR strips themselves, for...
31 July 2024 2,406 6 View
Can anyone with an MJ research / BioRad PCR machine from ~2010 or earlier tell me the external measurements (LxWxH) of the removable standard PCR alpha modules that can be removed from a PTC200 or...
30 July 2024 2,867 0 View
I am attempting to isolate picocyanobacteria from seawater (pre-filtered with a GF/D membrane) using the pour plate technique and filter plating method (Kearney et al., 2022) on Pro99 and SNAX...
30 July 2024 1,040 1 View