I need to isolate DNA and further use it for PCR amplification purposes.
Does the ratio between mitochondrial fission vs fusion indicate mitochondrial biogenesis or is their a specific marker to anaylze mitochondrial biogenesis reliably? Looking forward to...
05 June 2018 4,041 4 View
I am trying to set up different assays to measure levels of reactive oxygen species. I would appreciate if anyone could share their experience regarding the usage of Dihydrorhodamine 123 to detect...
07 August 2017 7,641 3 View
I have two images of the same sample with different x,y,z dimensions. Can anyone explain - in order to quantify the same object - why do I need to enter larger number of voxels in the size filter...
05 June 2014 8,185 9 View
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08 September 2013 2,027 1 View
Even after specifying the flexible residues in the ligand file when I go ahead to save it as flexible pdbqt the prog says- " Must Specify Residues for flexible side chains or set hinge". Can...
03 April 2013 4,297 8 View
I am unable to find the ata related to the binding sites of my chosen protein. I have tried using Q Site Finder but its not helping much. Can anyone please suggest some other free online tools...
02 March 2013 1,979 15 View
Hello, We would like to increase the yield of our PCR product. We are running a series of PCR reactions that is targeting ~1.1kb sequence. We begin each reaction with ~400pg of template DNA...
02 March 2021 4,029 3 View
I am going to have 3 different probes in my qPCR work that I am going to do. But I realized that the machine we have in the lab is a Rotor-Gene Q 2plex HRM Platform, saying it has green, yellow,...
01 March 2021 8,544 1 View
To dear Researchers, I was analyzing a series of concentration for estimation of Real-Time PCR efficiency. The concentration was 1:10. I used MS-excel to evaluate Slope. The result of slope was -8...
01 March 2021 8,683 4 View
Does anyone have the experience of using Taq Man probes in the QIAGEN Rotar- Gene qPCR machine?
01 March 2021 5,311 1 View
Dear Dr. Cai, my name is Simone Prospero and I work in the team of Phytopathology at the Swiss Federal Institute for Forest Snow and Landscape Research (WSL;...
01 March 2021 1,133 1 View
Incompletely cross-linked pdms are difficult to remove.
01 March 2021 798 3 View
Dear All, mirna primer showing some problem in the melting curve? any idea why? As attached is the melting curve. The forward sequence is obtained from miRBase and reverse primer is universal.
28 February 2021 5,008 4 View
Is the period to autoclave not enough? The inoculation in a hood and flame and UV and alcohol.
27 February 2021 9,356 3 View
I performed site directed mutagenesis, transformation, and then I sent out plasmids for Sanger sequencing and found out that there is extension of DNA just before the stop codon. I am not sure...
27 February 2021 547 3 View
Hi, my question is about the heating of thermal cycler machines and I hope some of you had experienced a similar thing previously. There are two thermal cycler machines in the lab(BioRad) and for...
26 February 2021 4,777 4 View