Is there a marker for mitochondrial biogenesis ?

Does the ratio between mitochondrial fission vs fusion indicate mitochondrial biogenesis or is their a specific marker to anaylze mitochondrial biogenesis reliably? Looking forward to...

05 June 2018 4,041 4 View

Can anyone help with a 3D object counter plugin of ImageJ?

I have two images of the same sample with different x,y,z dimensions. Can anyone explain - in order to quantify the same object - why do I need to enter larger number of voxels in the size filter...

05 June 2014 8,185 9 View

Is there any bioinformatics tool available to calculate half life of mRNA?

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08 September 2013 2,027 1 View

Error while saving Flex file for Ligand when using Autodock - can anyone help?

Even after specifying the flexible residues in the ligand file when I go ahead to save it as flexible pdbqt the prog says- " Must Specify Residues for flexible side chains or set hinge". Can...

03 April 2013 4,297 8 View

How to find out the binding sites in a given protein?

I am unable to find the ata related to the binding sites of my chosen protein. I have tried using Q Site Finder but its not helping much. Can anyone please suggest some other free online tools...

02 March 2013 1,979 15 View

Can anyone suggest an optimized protocol for DNA isolation from Filamentous cyanobacteria, Wetsiellopsis?

I need to isolate DNA and further use it for PCR amplification purposes.

02 March 2013 1,622 0 View

How to quantitative analize the data of TNBS assay for gelatin?

Estemeed colleagues, I found some issues regarding the quantification of the data for TNBS assay. There are different protocols on how to perform that but it is clear to me the "fil rouge" that...

02 March 2021 2,616 1 View

How to simultaneously perform multiple biochemical assays?

I am a research scholar working on heavy metal stress on plants. I will be doing biochemical characterisation( protein, carbohydrate, proline, antioxidant enzymes and many more assays) at interval...

01 March 2021 6,999 3 View

Which fluorescent dye to use for measuring total ROS production?

Is DCFDA appropriate for measuring total ROS production, or can it only detect certain types of ROS? If I want to measure total ROS production, which dye should I use?

01 March 2021 9,447 3 View

Is CD44 degraded in lysosome together with hyaluronan after CD44 mediated endocytosis of hyaluronic acid?

Also when RHAMM binds hyaluronic acid, they get internalized, will RHAMM also be degraded? Or both CD44 and RHAMM will be transferred back to the cell membrane? Asking for breast cancer cell line...

01 March 2021 8,169 2 View

RIA. How to measure?

When you use RIA, with a control the unknown sample with antibodies, you bare in mind the binding sites of the antibodies. However you still need to measure labelled antigen (radioactive) and not...

28 February 2021 2,133 3 View

How to Eliminate cosine error and abbe error in LASER measurement system(for example Rhenishaw XL80)?

I like to do project in laser measurement system. My objective is to eliminate or reduce the cosine error, abbe error in Laser measurement system. I have Rhenishaw XL80 system. Please tell the...

28 February 2021 4,890 3 View

Can anyone help with Schistosoma identification?

Can anyone identify what kind of Schistosome species this might be? Morphological features of cercariae and adults resemble Trichobilharzia, though I am fairly new to the field of parasitology...

25 February 2021 7,760 6 View

How to measure angle between laser travel path and machine axis in Laser Measurement system?

25 February 2021 1,987 3 View

I have a time series data of some biochemical studies ( DNA and Chromosomal damage) which I intend to use to further predict into future ?

I have a time series data of some biochemical studies ( DNA and Chromosomal damage) which I intend to use to further predict into future without necessarily conducting the assay for an extended...

23 February 2021 7,842 1 View

What is the standard protocol in doing replicates for C. elegans lifespan assay on solid media (thru worm picking)?

I would like to know what is the standard protocol in the number of worms per plate and number of plates per concentration of extract X in a lifespan assay done in solid media and not automated...

23 February 2021 9,933 1 View