I want to determine the antibacterial activity of Zinc Oxide nanoparticles by the disc diffusion method. In order to impregnate the disc with the appropriate concentration of ZnO, it has to be diluted (not dissolved) homogeneously. I have used methanol, DCM, and DMSO. I also used sonication and vortex shaking for the homogenization. Nevertheless, the ZnO did not show any antibacterial activity. Can anyone provide me a detailed SOP or procedure and suggest me a suitable solvent? Thanks in advance.
Dear all, most investigations use double distilled water. Please check the following free documents. My Regards
DOI: 10.1128/AEM.02149-10
https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6223899/
https://www.scielo.br/scielo.php?script=sci_arttext&pid=S0104-66322019000200885
https://www.researchgate.net/publication/323792188_Mechanistic_study_on_antibacterial_action_of_zinc_oxide_nanoparticles_synthesized_using_green_route
Dear Sajib Aninda Dhar;
the solution of ZnO NPs (200 mg mL1) can be prepared by dissolving 20 g of ZnO NPs in 100 mL of sterile distilled water; including 4 g of Polystyrene sulfonate PSS to enhance the stability of the ZnO NPs in the solution. you can also use, acetone or 25% ethanol, to suspend ZnO nanoparticles.
Dear all, most investigations use double distilled water. Please check the following free documents. My Regards
DOI: 10.1128/AEM.02149-10
https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6223899/
https://www.scielo.br/scielo.php?script=sci_arttext&pid=S0104-66322019000200885
https://www.researchgate.net/publication/323792188_Mechanistic_study_on_antibacterial_action_of_zinc_oxide_nanoparticles_synthesized_using_green_route
Dear Sajib Aninda Dhar
Please follow these below links. It may helpful for you!
Article A Facile Approach for the Preparation of Nano-size Zinc Oxid...
https://www.researchgate.net/post/How-to-dissolve-ZnO-nanoparticles-in-water-or-suggest-any-other-solvent-to-dissolve-ZnO
https://www.researchgate.net/post/Which_solvent_is_suitable_to_dissolve_Copper_and_Zinc_oxide_nano_particles
https://www.researchgate.net/post/How_can_I_completely_dissolve_ZnO_and_TiO2_Nanoparticles
https://www.longdom.org/open-access/synthesis-and-characterizations-of-zinc-oxide-nanoparticles-forantibacterial-applications-2157-7439-S8-004.pdf
- Badges
- Science topic