Which are the premier Digital Health Journals?

Hei! I am trying to understand the landscape of Digital Health Journals. Is this list below capturing the main (and premier) journals? - Digital Health (Sage) - NPJ Digital Medicine - Lancet...

19 November 2020 4,616 4 View

What does it mean if an anaplastic thyroid cancer has no mutations or fusions in a comprehensive PCR analysis?

The tumor has p53 possitivity, but a comprensive PCR analysis showed no mutations in tp53.

30 March 2020 884 3 View

Meta-analysis on prognostic studies: How to reach an optimal cut-off value?

I'm doing a meta-analysis on prognostic studies. The studies I have included present the following values: AUC, 95% CI, a cut-off value and associated sensitivity and specificity. Also, is it...

01 March 2020 10,001 2 View

Can anyone suggest a robust ELISA kit for Lipopolysaccharide(LPS)-binding protein (LBP) detection?

I am in the process of recruiting patients to a study investigating bacteria and inflammation in the body of children. As part of this study, I am interested in comparing the quantity of LPS...

01 February 2019 1,590 2 View

How can I quantify the intensity of fluorescence in my .TIFF confocal images?

I am currently writing my first master project. I have several images of tissue stained with Alexa Fluor 488, and I need to determine difference in fluorescence intensity between my control and...

11 December 2017 2,733 4 View

How to culture cells before alizarin red staining.?

08 May 2017 5,630 7 View

No title

I am investigating the predictors for adverse events (AEs) from a sample of 400 patients recieving an injection therapy. The outcome AEs is binary and I will use a multiple logistic regression...

01 June 2016 5,727 3 View

Recovery of freeze damaged muscle tissue sections on glass slides?

04 April 2016 5,142 5 View

Formation of strong green background fluorescence following a few days after staining?

I have been doing some ERBB2 / CEN17 FISH on breast carcinomas recently. We obtained good images in both the red (Ex: 535-555 nm; em: 570-625 nm)(CEN17) and green channel (Ex: 470-495 nm; Em:...

01 February 2014 7,705 33 View

Highly specific FISH probes for HPV detection - Is it possible to use oligonucleotide probes?

We are trying to detect integration of HPV (human papillomavirus) sequences from specific HPV types in the genome of epithelial cells. The problem here, is that there are quite large similarities...

11 December 2013 939 4 View

Enhanced Yellow Fluorescent Protein (Aequorea victoria) DNA sequence?

I am on the lookout for the Enhanced Yellow Fluorescent Protein (Aequorea victoria) DNA sequence. Does anyone know where I can find it? Thank you in advance

03 March 2021 3,568 1 View

What are the values needed for gabor filter's parameter for cifar10 data?

Hi, I implemented a code to gabor filter cifar10 data but the images after being filtered and stacked are not clear like the original images. I think the problem is in the way I am using the...

03 March 2021 6,317 1 View

What is the most reliable source to get Impact factor of journals?

Source to get Impact factor of journals

01 March 2021 6,654 2 View

Which index or sites are reliable in determining of plant species?

There are many indexes when determining plant species: Plants of the World Online (POWO), International Plants Names Index (www.ipni.org), The Plant List (www.theplantlist.org) and others. But...

26 February 2021 3,404 10 View

What's the concentration recommend for recombinant protein use in western blotting?

I'm currently working having problem with the concentration for recombinant protein use in western blot, the one been used is Recombinant Human CD19 Fc Chimera Protein-rndsystem which does not...

24 February 2021 8,514 3 View

How do historians determine the credibility sources in modern history?

For a source to be considered reliable it must contain accurate historical information. But how does one determine the accuracy and reliability of sources in modern and recent history since not...

23 February 2021 1,346 13 View

Three-way robust ANOVA in R?

I am trying to conduct a three-way robust ANOVA in R using the WRS2 package as: t3way(Stem ~ Species*Temperature*Salinity, data = tutto) and there is an error saying : Error in if...

22 February 2021 1,674 8 View

Interpretation of Goodman - Kruskal Gamma squared?

Dear colleages, Eta squared is dominantly used in reflecting the explaining power the same manner as a squared partial correlation coefficient (R2p ) from multiple linear regression: as the...

22 February 2021 7,801 3 View

Will SARS-CoV-2 Spike be broken into S1 and S2 by denaturing conditions?

Hello, I am performing a western blot of my SARS-CoV-2 Spike-pseudotyped lentiviral particles. I denature my virus by adding concentrated virus to 2X Laemmli buffer at 1:1, then boiling for...

17 February 2021 4,844 4 View

How can we measure the reliability and validity of our survey questionnaire?

We are conducting survey research on COVID-19 misinformation among students. How can we validate our survey for it to produce valid and reliable data? Also, what statistical tools can we use to...

16 February 2021 678 3 View