Can you recommend me some articles that dealing with the electronic transitions of toluene?

Hello! I'm working on assigning absorption bands to UV-vis spectra of unknown matrix. I think some are due to the presence of toluene, but I can't find any article that describes the electronic...

19 March 2019 8,119 3 View

I'll work with strict anaerobes (without an anaerobic chamber). Are the anaerobic jars and the pre-reduction of the media enough?

I am wondering if I can do the manipulation of the microorganisms on the bench and then incubate them anaerobically on pre-reduced media in jars, including an anaerobic generator bag. Will a brief...

08 March 2018 6,217 5 View

Does someone knows how to wash or clean SPEs (Drop Sense minielectrodes)?

Hello! I'd like to have some info about some DS Drop screen printed electrodes (DS 110, 220AT and 550) concerning the way of washing them and the no of cycles each one can be used (in aqueous...

24 October 2016 2,023 3 View

Any advice in replacing missing values?

Hi Everyone, To be honest I have never heard of or used this function in SPSS. However some fellow researchers told me that it is often used by researchers and it is encouraged by scientific...

08 October 2015 6,649 36 View

Could anyone recommend a stable high transfection efficiency mouse cell line that I could use?

I've been trying to transfect MEF cells using several methods and I did not get a very high efficiency (we have the Nucleofection kit too but there are many constructs that I need to transfect and...

04 May 2014 6,736 9 View

Could anyone recommend some methods (e.g. staining) for testing if cultured MEF cells are indeed MEF cells?

I have been culturing MEF cells and tried to use different transfection methods to transfect them and they show very low efficiency and we would like to make sure that they are indeed MEFs so that...

27 April 2014 8,403 3 View

Is there a commercially available antibody against Influenza A/California/04/2009 neuraminidase?

Does anyone know of a commercially available source for an anti-neuraminidase antibody for Influenza A/California/04/2009 suitable for use in Western blotting (either reducing or non-reducing)?

21 April 2014 6,474 3 View

Design thinking in academic research?

01 January 1970 3,275 11 View

What is the production process of CaFe ferroalloys?

I looking for ferro calcium production process

03 March 2021 1,755 4 View

How to quantitative analize the data of TNBS assay for gelatin?

Estemeed colleagues, I found some issues regarding the quantification of the data for TNBS assay. There are different protocols on how to perform that but it is clear to me the "fil rouge" that...

02 March 2021 2,616 1 View

How to simultaneously perform multiple biochemical assays?

I am a research scholar working on heavy metal stress on plants. I will be doing biochemical characterisation( protein, carbohydrate, proline, antioxidant enzymes and many more assays) at interval...

01 March 2021 6,999 3 View

Is CD44 degraded in lysosome together with hyaluronan after CD44 mediated endocytosis of hyaluronic acid?

Also when RHAMM binds hyaluronic acid, they get internalized, will RHAMM also be degraded? Or both CD44 and RHAMM will be transferred back to the cell membrane? Asking for breast cancer cell line...

01 March 2021 8,169 2 View

RIA. How to measure?

When you use RIA, with a control the unknown sample with antibodies, you bare in mind the binding sites of the antibodies. However you still need to measure labelled antigen (radioactive) and not...

28 February 2021 2,133 3 View

Is there a fast method matching citations to bibliography (manually typed references)?

I need to be able to match in-text citations to a reference list and back again in very large documents (100+ pages) WITHOUT the use of referencing software like Endnote. my method is to type the...

27 February 2021 9,848 1 View

How to empty large intestine in mice ?

Hi all I am working on colon-targeted drug delivery for which I kept mice fasting for 6h using fasting grills during scotophase (dark period). I observed liquid fecal content in the cecum and...

23 February 2021 7,701 2 View

Best plasmid could be used as a standard curve for AAV titration?

I have been trying to have a good AAV titration for my production and I have tried different plasmids to set up as a standard curve but each one behaves differently. i am trying to use ITR for...

23 February 2021 5,167 3 View

I have a time series data of some biochemical studies ( DNA and Chromosomal damage) which I intend to use to further predict into future ?

I have a time series data of some biochemical studies ( DNA and Chromosomal damage) which I intend to use to further predict into future without necessarily conducting the assay for an extended...

23 February 2021 7,842 1 View

What is the standard protocol in doing replicates for C. elegans lifespan assay on solid media (thru worm picking)?

I would like to know what is the standard protocol in the number of worms per plate and number of plates per concentration of extract X in a lifespan assay done in solid media and not automated...

23 February 2021 9,933 1 View