Dora Akritidou

5 Questions 5 Answers 0 Followers

Questions related from Dora Akritidou

How can I tackle the antimicrobial effect of LCFAs?

I aim to cultivate a mixed culture of bacteria anaerobically in a medium that contains LCFAs (15 g/L oleic acid and 30 g/L palmitic acid), pH 7.2. The medium contains peptone and fibers as well,...

09 March 2020 5,846 2 View

How can I store enzymes in a solution and maintain their activity?

I work with a-amylase, pepsin and pancreatin and I have a powder stock stored at -20oC. Can I prepare enzyme solutions and store them in the freezer and how? Do I need to add glycerol or just MQ...

22 March 2019 4,021 6 View

Can anyone suggest a protocol for measuring the calcium (Ca 2+) content of Pancreatin?

All I find in literature so far are assay kits and titration methods based on EDTA/MgCl2. Which is the most fast, accurate and non expensive method?

03 December 2018 4,829 3 View

Do I need to conduct enzymatic assays on digestive enzymes to determine their specific activity, every time before the in vitro simulation?

I am going to do in-vitro simulations of the human digestion process, so I am planning to include all the relevant enzymes. My question is, are the enzymatic assays required after I buy the...

14 May 2018 1,930 4 View

I'll work with strict anaerobes (without an anaerobic chamber). Are the anaerobic jars and the pre-reduction of the media enough?

I am wondering if I can do the manipulation of the microorganisms on the bench and then incubate them anaerobically on pre-reduced media in jars, including an anaerobic generator bag. Will a brief...

09 March 2018 6,360 5 View