Can you recommend me some articles that dealing with the electronic transitions of toluene?

Hello! I'm working on assigning absorption bands to UV-vis spectra of unknown matrix. I think some are due to the presence of toluene, but I can't find any article that describes the electronic...

19 March 2019 8,119 3 View

I'll work with strict anaerobes (without an anaerobic chamber). Are the anaerobic jars and the pre-reduction of the media enough?

I am wondering if I can do the manipulation of the microorganisms on the bench and then incubate them anaerobically on pre-reduced media in jars, including an anaerobic generator bag. Will a brief...

08 March 2018 6,217 5 View

Does someone knows how to wash or clean SPEs (Drop Sense minielectrodes)?

Hello! I'd like to have some info about some DS Drop screen printed electrodes (DS 110, 220AT and 550) concerning the way of washing them and the no of cycles each one can be used (in aqueous...

24 October 2016 2,023 3 View

Any advice in replacing missing values?

Hi Everyone, To be honest I have never heard of or used this function in SPSS. However some fellow researchers told me that it is often used by researchers and it is encouraged by scientific...

08 October 2015 6,649 36 View

Could anyone recommend a stable high transfection efficiency mouse cell line that I could use?

I've been trying to transfect MEF cells using several methods and I did not get a very high efficiency (we have the Nucleofection kit too but there are many constructs that I need to transfect and...

04 May 2014 6,736 9 View

Could anyone recommend some methods (e.g. staining) for testing if cultured MEF cells are indeed MEF cells?

I have been culturing MEF cells and tried to use different transfection methods to transfect them and they show very low efficiency and we would like to make sure that they are indeed MEFs so that...

27 April 2014 8,403 3 View

Is there a commercially available antibody against Influenza A/California/04/2009 neuraminidase?

Does anyone know of a commercially available source for an anti-neuraminidase antibody for Influenza A/California/04/2009 suitable for use in Western blotting (either reducing or non-reducing)?

21 April 2014 6,474 3 View

Design thinking in academic research?

01 January 1970 3,275 11 View

Does Napabucasin soluble in dichloromethane and in ethanol?

I need to prepare a solution of Napabucasin (inhibitor of cancer cell stemness) in dichloromethane. The drug is expensive, so maybe somebody already tried to do it. If it is soluble, what is the...

02 March 2021 9,945 3 View

Is CD44 degraded in lysosome together with hyaluronan after CD44 mediated endocytosis of hyaluronic acid?

Also when RHAMM binds hyaluronic acid, they get internalized, will RHAMM also be degraded? Or both CD44 and RHAMM will be transferred back to the cell membrane? Asking for breast cancer cell line...

01 March 2021 8,169 2 View

Unknown debris sheets observed in cell culture flasks?

Hi, We culture neural progenitor cells in T175 culture flasks coated with PLO-Laminin. We have successfully grown these cells with our protocol for months. Recently, we observed large sheets...

25 February 2021 3,443 4 View

Oil red O protocol for flow cytometry?

18 February 2021 4,214 3 View

Which detection method is better for an ELISA-based assay: colorimetric or fluorometric?

Hello, I am planning to perform an ELISA-based assay to quantify the percentage of genomic methylation (5-methylcytosine) in human embryonic stem cells. I've been searching for some kits and I...

16 February 2021 8,389 3 View

Is my GFAP staining in right position?

I was differentiate hESC to astrocyte. In the second week of differentiation, I stained my cell with s100beta and GFAP. I expected that the staining site of GFAP would come from the cytoplasm,...

15 February 2021 8,982 1 View

Hi, I have a problem with my mouse Adipose MSC primary culture?

I am working on mouse adipose mesanchymal stem cells. After removing the mouse peritoneal fat and extracting, I do the primary culture, I used DMEM F12 medium with 15% FBS, but with this, the...

13 February 2021 9,838 1 View

Which is the best way to calculate doubling time for human embryonic stem cells?

Hi! I would like to perform a doubling time assay in several newly derived human embryonic stem cell lines. Since hESC must be passaged as cell clumps, I cannot know the exact number of cells I am...

10 February 2021 2,895 4 View

What is the main difference between "CD105+ Endothelial Cells" and mesenchymal stem cells?

since CD105+ Endothelial Cells are CD31 negative, how do we differentiate them in cell culture from mesenchymal cells? is it using vwf? thanks a lot

03 February 2021 5,362 2 View

What is the difference between human umbilical vein endothelial cells (HUVECs) and bone marrow endothelial cells?

Hello I am new to work on endothelial cells, and I have been reading about them a lot and trying to know the difference between human umbilical vein endothelial cells (HUVECs) and bone marrow...

28 January 2021 8,765 4 View