You could try the ethanol-nitric  acid method (Kurschner-Hoffer, 1931)  but there are also a few other methods such as NREL methods acetylacetone method....I've personally used the Kurschner-Hoffer with good reproductibility results on straws and other nonwoods...

Putel advice on this is correct. Also, go though the Tappi standard method for Alfa cellulose

The common methods for measuring cellulose, hemi-cellulose and lignin are Neutral Detergent fiber (NDF), Acid Detergent Fiber (ADF) and 72% sulfuric Acid lignin (ADL).  You can see method at these links

https://www.ankom.com/sites/default/files/document-files/Method_13_NDF_A2000.pdf

https://www.ankom.com/sites/default/files/document-files/Method_12_ADF_A2000.pdf

https://www.ankom.com/sites/default/files/document-files/Method_8_Lignin_in_beakers.pdf

The analysis, porosity, crystallinity, and reactivity of never-dried cellulose fibers in green unopened cotton bolls are discussed in the below mentioned articles. Happy new year.

https://www.researchgate.net/publication/312695203_On_fine_structure_of_cellulose_fibers?_sg=gMfP5CcIeMtf8Ui1o2D3FcfifSd-jir1GBvosgkmxo4A8We9XFQwCIfRFn1pzs1by7vyJTa0sbnrQ1xzC4F_-rJPLyqkQUE04RNdPfWO.p5kh-DbWyXujNy9tZupEqqlEFISvwAfpAQS7gxv5QQb6LoNuY77hWx1CJaLD2_Puf4MN-E19g5ZJ51ZcP27ceg

https://www.researchgate.net/publication/250425822_Fine_structure_and_reactivity_of_cellulose?_sg=5fWap_Bo_4zdD_fvqH_ik_rICR79B2MqsJkQJ-GpEk0-kWUql-OagXiQ1htoCGtqy5kKGHOkgcXg6Iwn4D16loYczMtz8vJCHsSulhkH.IexQikLYtmzkEXZKF7V0Hp1o92stM2-UVAXYD-fAlxbH24tcvwxDuP_sMPad0e1_V30QOrezdoD_Gdr1Cdoxsw

https://www.researchgate.net/publication/298681455_Reactivity_of_biological_cellulose_and_properties_of_some_of_its_derivatives?_sg=5fWap_Bo_4zdD_fvqH_ik_rICR79B2MqsJkQJ-GpEk0-kWUql-OagXiQ1htoCGtqy5kKGHOkgcXg6Iwn4D16loYczMtz8vJCHsSulhkH.IexQikLYtmzkEXZKF7V0Hp1o92stM2-UVAXYD-fAlxbH24tcvwxDuP_sMPad0e1_V30QOrezdoD_Gdr1Cdoxsw

https://www.researchgate.net/publication/294690836_On_the_properties_of_never-dried_and_nature-dried_cotton?_sg=5fWap_Bo_4zdD_fvqH_ik_rICR79B2MqsJkQJ-GpEk0-kWUql-OagXiQ1htoCGtqy5kKGHOkgcXg6Iwn4D16loYczMtz8vJCHsSulhkH.IexQikLYtmzkEXZKF7V0Hp1o92stM2-UVAXYD-fAlxbH24tcvwxDuP_sMPad0e1_V30QOrezdoD_Gdr1Cdoxsw

https://www.researchgate.net/publication/312702652_On_the_activation_of_cellulose_through_xanthation_II_Viscose_and_rayon_from_activated_cellulose?_sg=5fWap_Bo_4zdD_fvqH_ik_rICR79B2MqsJkQJ-GpEk0-kWUql-OagXiQ1htoCGtqy5kKGHOkgcXg6Iwn4D16loYczMtz8vJCHsSulhkH.IexQikLYtmzkEXZKF7V0Hp1o92stM2-UVAXYD-fAlxbH24tcvwxDuP_sMPad0e1_V30QOrezdoD_Gdr1Cdoxsw

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Source

Article: On fine structure of cellulose fibers

Y Fahmy · F Mobarak

Full-text available · Article · Jan 1971 · Svensk Papperstidning

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Adrian Catalin.

I want to use the Kurschner-Hoffer method for cellulose analysis. Could you say me what perils have in this method? Warnings about nitrated material maybe.

Please would you send me the Kurschner-Hoffer method that use?

Dear victor

if you take a look at the old resesrch articles of the below mentioned reseacher, you will find several standard methods for cellulose determination in non-wood and wood fibers. Happy new year.

https://www.researchgate.net/profile/Yehia_Fahmy

Thanks, Tamer YA Fahmy.

I revised all but I want to use the Kurschner-Hoffer method. I read "Care should be taken no to allow the alcohol in the filter flask to evaporate to dryness, as some of the nitrated material may be explosive" ref.

Cellulose Analysis

J. David Reid and D. F. J. LynchIndustrial & Engineering Chemistry Analytical Edition 1937 9 (12), 570-573DOI: 10.1021/ac50116a010

I do not know if more perils or warnings to consider

I agree with Dr. Bharath's suggestion! In my opinion it is a fairly reliable method, since it considers the characteristics of the raw material analyzed.

Thanks

I also agree the TAPPI is the most adequatte method. NREL

The TAPPI standards are suitable for any lignocellulosic material.

Adrian Catalin Puitel Im interested in Kurschner-Hoffer, 1931 method, may i know at what concentration of ethanol and nitric acid should I used to determine cellulose content?

Yes. I Agree

TAPPI Standard used to determined the chemical content of four woody trees

property

Standard method

Sample preparation

TAPPI 264 cm 97

Moisture content

TAPPI 264 om-88

Ash content

TAPPI 211 om 93

Hot water extraction

TAPPI 207 cm 99

1% sodium hydroxide extraction

TAPPI 212 om 02

Alcohol-benzene extraction

TAPPI 204 cm 97

Cellulose

Kurchner-Hoffer

Pentosan

TAPPI 223 cm-01

Lignin

TAPPI 222 om-02

Alpha cellulose

TAPPI 203 cm-99