Can anyone suggest a good protein cell fractionation kit?

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Anyone know a good anti-phosphothreonine and anti-phosphoserine antibodies for IP and WB?

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Co-IP using crosslinking

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Hiseq2000 vs Miseq What are the differences??

What do you choose for ChIP-seq? Thanks!

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Will a 10xHis-tag prevent anti-MBP from binding to MBP?

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What's the concentration recommend for recombinant protein use in western blotting?

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What is the best way to manage Western blots?

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How to manage western blot for proteomics data validation?

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Diltuions for a Western blot?

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Can anyone help me how to get the similar bands in Actin?

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I see 2 random bands in my ERK/pERK blot? Has anyone else seen this?

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Will SARS-CoV-2 Spike be broken into S1 and S2 by denaturing conditions?

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(western blot) can methanol damage my antibody signal?

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Jorge Martin-Perez

Loreto we were recommended by the Proteomic Service of the “Centro Nacional de Biotecnologia” (Consejo Superior de Investigaciones Cientificas, Madrid) to use ProteoExtract (Subcellular Proteome Extraction Kit) from Calbiochem (Cat# 539790). It is clear that when you collect the “membrane fraction”, it contains cellular membranes and not only plasma membrane. I hope this information is useful for you.

Best regards,

Jorge

Loreto Carvallo

yes please! I appreciate!!! my email is [email protected]

and Do you know how it work (in the another lab)???